Endogenous Biosynthesis of S-Nitrosoglutathione From Nitro-Fatty Acids in Plants

Frontiers in Plant Science

Antonio Molina-Díaz¹  David Moreno-González¹  Lorena Aranda-Caño²  Beatriz Sánchez-Calvo²  Raquel Valderrama²  Mounira Chaki²  Juan C. Begara-Morales²  Juan B. Barroso²  Capilla Mata-Pérez²  María N. Padilla² 

[1] Analytical Chemistry Research Group, Department of Physical and Analytical Chemistry, University of Jaén, Jaén, Spain;Group of Biochemistry and Cell Signalling in Nitric Oxide, Department of Experimental Biology, Center for Advanced Studies in Olive Grove and Olive Oils, Faculty of Experimental Sciences, University of Jaén, Jaén, Spain;

关键词: nitro-fatty acids;    nitric oxide;    S-nitrosoglutathione;    S-nitrosothiols;    NO-signaling;    nitric oxide donor;   

DOI  :  10.3389/fpls.2020.00962

来源: DOAJ

【 摘 要 】

Nitro-fatty acids (NO2-FAs) are novel molecules resulting from the interaction of unsaturated fatty acids and nitric oxide (NO) or NO-related molecules. In plants, it has recently been described that NO2-FAs trigger an antioxidant and a defence response against stressful situations. Among the properties of NO2-FAs highlight the ability to release NO therefore modulating specific protein targets through post-translational modifications (NO-PTMs). Thus, based on the capacity of NO2-FAs to act as physiological NO donors and using high-accuracy mass-spectrometric approaches, herein, we show that endogenous nitro-linolenic acid (NO2-Ln) can modulate S-nitrosoglutathione (GSNO) biosynthesis in Arabidopsis. The incubation of NO2-Ln with GSH was analyzed by LC-MS/MS and the in vitro synthesis of GSNO was noted. The in vivo confirmation of this behavior was carried out by incubating Arabidopsis plants with 15N-labeled NO2-Ln throughout the roots, and 15N-labeled GSNO (GS15NO) was detected in the leaves. With the aim to go in depth in the relation of NO2-FA and GSNO in plants, Arabidopsis alkenal reductase mutants (aer mutants) which modulate NO2-FAs levels were used. Our results constitute the first evidence of the modulation of a key NO biological reservoir in plants (GSNO) by these novel NO2-FAs, increasing knowledge about S-nitrosothiols and GSNO-signaling pathways in plants.

【 授权许可】

Unknown