期刊论文详细信息
Electronic Journal of Biotechnology
Development of Kompetitive Allele Specific PCR markers for identification of persimmon varieties using genotyping-by-sequencing
Ye-Seul Jo1  Sang-Jin Yang2  Moon Nam2  Kyeong Bok Ma3  Sam Seok Kang3 
[1] Corresponding author.;Herbal Science, 121, Byeongnyugil, Geumcheon-Myeon, Naju-si, Jeollanam-do 58216, Republic of Korea;;Pear Research Institute, National Institute of Horticultural &
关键词: Accession;    Genotyping-by-sequencing;    Homozygous;    KASP markers;    Kompetitive Allele;    Specific PCR;   
DOI  :  
来源: DOAJ
【 摘 要 】

Background: Persimmon (Diospyros kaki Thunb.) is the most widely cultivated species of the genus Diospyros. In this study, genetic diversity and variations in persimmon genotypes were investigated using single nucleotide polymorphism (SNP) markers identified by genotyping-by-sequencing (GBS) analysis. Results: Ninety-five persimmon accessions grown in the Pear Research Institute, National Institute Horticultural and Herbal Science, were sequenced using the Illumina Hiseq2500 platform and polymorphic SNPs were detected to develop molecular markers. These reliable SNPs were analyzed using the Kompetitive Allele Specific PCR (KASP) assay to discriminate among persimmon genotypes. GBS generated a total of 447,495,724 trimmed reads, of which 89.7% were raw reads. After demultiplexing and sequence quality trimming, 108,876,644 clean reads were mapped to the reference transcriptome. An average of 1,146,070 genotype reads were mapped. Filtering of raw SNPs in each sample led to selection of a total of 1,725,401 high-quality SNPs. The number of homozygous and heterozygous SNPs ranged from 1,933 to 6,834 and from 846 to 5,927, respectively. Conclusions: Of the 49 SNPs selected for development of an identification system for persimmons, 15 SNPs were used in the KASP assay to analyze 32 persimmon accessions. These KASP markers discriminated among all accessions.How to cite: Ma KB, Yang SJ, Jo YS, et al. Development of Kompetitive Allele Specific PCR markers for identification of persimmon varieties using genotyping-by-sequencing. Electron J Biotechnol 2021;49.https://doi.org/10.1016/j.ejbt.2020.11.003

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