Genetics and mapping of a new anthracnose resistance locus in Andean common bean Paloma

BMC Genomics

Research Article

Marta Zulema Galván¹  Giselly Figueiredo Lacanallo²  Maria Celeste Gonçalves-Vidigal²  Thiago Alexandre Santana Gilio²  Vanusa da Silva Ramos Martins²  Sandra Aparecida de Lima Castro²  Giseli Valentini²  Qijian Song³  Oscar P. Hurtado-Gonzales³  Marcial Antonio Pastor-Corrales³ 

[1] CONICET, Laboratorio de Biotecnología, Estación Experimental Agropecuaria Salta, Instituto Nacional de Tecnología Agropecuaria, Cerrillos, Salta, Argentina;Departamento de Agronomia, Universidade Estadual de Maringá, Maringá, Paraná, Brazil;Soybean Genomics and Improvement Laboratory, United States Department of Agriculture, Agricultural Research Service, Beltsville, MD, USA;

关键词: Phaseolus vulgaris;    Colletotrichum lindemuthianum;    Genetic resistance;    KASP markers;    SNP markers;   

DOI  :  10.1186/s12864-017-3685-7

 received in 2016-11-04, accepted in 2017-04-06,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundThe Andean cultivar Paloma is resistant to Mesoamerican and Andean races of Colletotrichum lindemuthianum, the fungal pathogen that causes the destructive anthracnose disease in common bean. Remarkably, Paloma is resistant to Mesoamerican races 2047 and 3481, which are among the most virulent races of the anthracnose pathogen. Most genes conferring anthracnose resistance in common bean are overcome by these races. The genetic mapping and the relationship between the resistant Co-Pa gene of Paloma and previously characterized anthracnose resistance genes can be a great contribution for breeding programs.ResultsThe inheritance of resistance studies for Paloma was performed in F2 population from the cross Paloma (resistant) × Cornell 49–242 (susceptible) inoculated with race 2047, and in F2 and F2:3 generations from the cross Paloma (resistant) × PI 207262 (susceptible) inoculated with race 3481. The results of these studies demonstrated that a single dominant gene confers the resistance in Paloma. Allelism tests performed with multiple races of C. lindemuthianum showed that the resistance gene in Paloma, provisionally named Co-Pa, is independent from the anthracnose resistance genes Co-1, Co-2, Co-3, Co-4, Co-5, Co-6, Co-12, Co-13, Co-14, Co-15 and Co-16. Bulk segregant analysis using the SNP chip BARCBean6K_3 positioned the approximate location of Co-Pa in the lower arm of chromosome Pv01. Further mapping analysis located the Co-Pa gene at a 390 kb region of Pv01 flanked by SNP markers SS82 and SS83 at a distance of 1.3 and 2.1 cM, respectively.ConclusionsThe results presented here showed that Paloma cultivar has a new dominant gene conferring resistance to anthracnose, which is independent from those genes previously described. The linkage between the Co-Pa gene and the SS82 and SS83 SNP markers will be extremely important for marker-assisted introgression of the gene into elite cultivars in order to enhance resistance.

【 授权许可】

CC BY   
© The Author(s). 2017