Demonstrating the presence of Ehrlichia canis DNA from different tissues of dogs with suspected subclinical ehrlichiosis

Parasites & Vectors

José J. Lira-Amaya¹  Julio V. Figueroa-Millán¹  Jesús A. Álvarez-Martínez¹  José A. Ibancovichi-Camarillo²  Jaime R. Adame-Gallegos³  Luis Soon-Gómez⁴  Ramón Rivera-Barreno⁵  Jane Tapia-Alanís⁵  Diana M. Beristain-Ruiz⁵  Federico Pérez-Casio⁵  Carlos A. Rodríguez-Alarcón⁵  Andrés Quezada-Casasola⁵  Orlando S. Cera-Hurtado⁵  Angélica Olivares-Muñoz⁶ 

[1] CENID-Salud Animal e Inocuidad. INIFAP;Department of Veterinary Anesthesia, Analgesia and Pharmacology, Faculty of Veterinary Medicine, Universidad Autónoma del Estado de México, El Cerrillo Piedras Blancas;Faculty of Chemistry, Universidad Autónoma de Chihuahua;Municipal Anti-Rabies Center, Jurisdicción Sanitaria II, Servicios de Salud de Chihuahua;Veterinary Science Department, Universidad Autónoma de Ciudad Juárez;Veterinary and Zootechnic Faculty, Universidad Veracruzana;

关键词: Ehrlichia canis;    Biopsies;    Spleen;    Bone marrow;    Liver;    Lymph node;   

DOI  :  10.1186/s13071-020-04363-0

来源: DOAJ

【 摘 要 】

Abstract Background Nowadays, Ehrlichia canis receives increasing attention because of its great morbidity and mortality in animals. Dogs in the subclinical and chronic phases can be asymptomatic, and serological tests show cross-reactivity and fail to differentiate between current and past infections. Moreover, there could be low parasitaemia, and E. canis might be found only in target organs, hence causing results to be negative by polymerase chain reaction (PCR) on blood samples. Methods We evaluated by PCR the prevalence of E. canis in blood, liver, spleen, lymph node and bone marrow samples of 59 recently euthanised dogs that had ticks but were clinically healthy. Results In total, 52.55% of the blood PCRs for E. canis were negative, yet 61.30% yielded positive results from tissue biopsies and were as follows: 63.15% from bone marrow; 52.63% from liver; 47.36% from spleen; and 15.78% from lymph node. In addition, 33% had infection in three tissues (spleen, liver and bone marrow). Conclusions Our results show the prevalence of E. canis from tissues of dogs that were negative by blood PCR. Ehrlichia canis DNA in tissue was 30% lower in dogs that tested negative in PCR of blood samples compared to those that were positive. However, it must be taken into account that some dogs with negative results were positive for E. canis in other tissues.

【 授权许可】

Unknown