Physicochemical Properties and Biocompatibility Evaluation of Collagen from the Skin of Giant Croaker (Nibea japonica)

Marine Drugs

Xiaoyan Li¹  Shujie Jin²  Fangfang Huang²  Zuisu Yang²  Guofang Ding²  Fangmiao Yu²  Xiaojuan Li²  Yan Chen²  Xuyang Hu²  Yunping Tang² 

[1] Hangzhou Meiya Pharmaceutical Co.Ltd, Hangzhou 310011, China;Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316022, China;

关键词: Nibea japonica;    collagen;    characterization;    biocompatibility;    cosmetics;   

DOI  :  10.3390/md16070222

来源: DOAJ

【 摘 要 】

Collagen and collagen peptides are widely used as cosmetic ingredients. In the present study, acid-solubilized collagen (ASC) and pepsin-solubilized collagen (PSC) were extracted from giant croaker (Nibea japonica) skin. The proline hydroxylation rates of ASC and PSC were 38.1% and 39.3%. The denaturation temperatures (Td) were approximately 34.5 °C for both ASC and PSC. The results of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and fourier transform infrared spetroscopy (FTIR) demonstrated that ASC and PSC were mainly type I collagen. Furthermore, As, Pb and Hg contents in the extracted collagen were lower than the national standards of China. In addition, collagen had good moisture absorption and retention properties when compared to glycerol. The collagen was also not cytotoxic to NIH-3T3 fibroblast cells, indicating that Nibea japonica skin collagen can be utilized in cosmetic applications.

【 授权许可】

Unknown