Association Mapping of Seedling Resistance to Tan Spot (Pyrenophora tritici-repentis Race 1) in CIMMYT and South Asian Wheat Germplasm

Frontiers in Plant Science

Muhammad Rezaul Kabir¹  Krishna Kanta Roy¹  Arun Kumar Joshi²  Santosh Kumar Bishnoi⁴  Gyanendra Pratap Singh⁴  Rahul Madhavrao Phuke⁵  Xinyao He⁶  Pawan Kumar Singh⁶  Philomin Juliana⁶  Ravi Prakash Singh⁶ 

[1] Bangladesh Wheat and Maize Research Institute, Dinajpur, Bangladesh;Borlaug Institute for South Asia, New Delhi, India;CIMMYT-India, New Delhi, India;ICAR- Indian Institute of Wheat and Barley Research, Karnal, India;ICAR-Indian Agriculture Research Institute, Regional Station, Indore, India;International Maize and Wheat Improvement Centre, Texcoco, Mexico;

关键词: tan spot;    genome wide association study;    seedling resistance;    Ptr race 1;    greenhouse screening;   

DOI  :  10.3389/fpls.2020.01309

来源: DOAJ

【 摘 要 】

Tan spot caused by Pyrenophora tritici-repentis (Ptr) is an important disease of wheat in many wheat producing areas of the world. A genome wide association study (GWAS) was conducted using 11,401 SNP markers of the Illumina Infinium 15K Bead Chip with whole genome coverage to identify genomic regions associated with resistance to tan spot in a diverse panel of 184 wheat genotypes originating from South Asia and CIMMYT. The GWAS panel was phenotyped for seedling resistance to tan spot with Ptr race 1 in two greenhouse experiments. Besides CIMMYT germplasm, several lines from South Asia (India, Bangladesh and Nepal) showed good degree of resistance to tan spot. Association mapping was conducted separately for individual experiments and for pooled data using mixed linear model (MLM) and Fixed and random model Circulating Probability Unification (FarmCPU) model; no significant MTAs were recorded through the MLM model, whereas FarmCPU model reported nine significant MTAs located on chromosomes 1B, 2A, 2B, 3B, 4A, 5A, 5B, 6A, and 7D. The long arms of chromosomes 5A and 5B were consistent across both environments, in which the Vrn-A1 locus was found in identified region of chromosome 5A, and MTA at IACX9261 on 5BL appears to represent the resistance gene tsn 1. MTAs observed on chromosomes 1B, 2A, 2B, 3B, 4A, 6A, and 7D have not been reported previously and are likely novel.

【 授权许可】

Unknown