Identification of leaf rust resistance loci in a geographically diverse panel of wheat using genome-wide association analysis

Frontiers in Plant Science

Plant Science

Rajeev Gupta¹  James A. Kolmer²  Harsimardeep S. Gill³  Sunish K. Sehgal³  Shivreet Kaur⁴  Upinder Gill⁴  Matthew Breiland⁴ 

[1] Cereal Crops Research Unit, Edward T. Schafer Agricultural Research Center, United States Department of Agriculture-Agricultural Research Service (USDA-ARS), Fargo, ND, United States;Cereal Disease Laboratory, United States Department of Agriculture-Agricultural Research Service (USDA-ARS), St. Paul, MN, United States;Department of Agronomy, Horticulture and Plant Science, South Dakota State University, Brookings, SD, United States;Department of Plant Pathology, North Dakota State University, Fargo, ND, United States;

关键词: leaf rust;    Puccinia triticana;    GWAS - genome-wide association study;    seedling resistance;    wheat;    QTL;    SNP;    marker assisted selection (MAS);   

DOI  :  10.3389/fpls.2023.1090163

 received in 2022-11-05, accepted in 2023-01-16,  发布年份 2023

来源: Frontiers

【 摘 要 】

Leaf rust, caused by Puccinia triticina (Pt) is among the most devastating diseases posing a significant threat to global wheat production. The continuously evolving virulent Pt races in North America calls for exploring new sources of leaf rust resistance. A diversity panel of 365 bread wheat accessions selected from a worldwide population of landraces and cultivars was evaluated at the seedling stage against four Pt races (TDBJQ, TBBGS, MNPSD and, TNBJS). A wide distribution of seedling responses against the four Pt races was observed. Majority of the genotypes displayed a susceptible response with only 28 (9.8%), 59 (13.5%), 45 (12.5%), and 29 (8.1%) wheat accessions exhibiting a highly resistant response to TDBJQ, TBBGS, MNPSD and, TNBJS, respectively. Further, we conducted a high-resolution multi-locus genome-wide association study (GWAS) using a set of 302,524 high-quality single nucleotide polymorphisms (SNPs). The GWAS analysis identified 27 marker-trait associations (MTAs) for leaf rust resistance on different wheat chromosomes of which 20 MTAs were found in the vicinity of known Lr genes, MTAs, or quantitative traits loci (QTLs) identified in previous studies. The remaining seven significant MTAs identified represent genomic regions that harbor potentially novel genes for leaf rust resistance. Furthermore, the candidate gene analysis for the significant MTAs identified various genes of interest that may be involved in disease resistance. The identified resistant lines and SNPs linked to the QTLs in this study will serve as valuable resources in wheat rust resistance breeding programs.

【 授权许可】

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Copyright © 2023 Kaur, Gill, Breiland, Kolmer, Gupta, Sehgal and Gill