Prediction of neuropeptide precursors and differential expression of adipokinetic hormone/corazonin-related peptide, hugin and corazonin in the brain of malaria vector Nyssorhynchus albimanus during a Plasmodium berghei infection

Current Research in Insect Science

María Carmen Rodríguez¹  Juan Téllez-Sosa¹  Everardo Gutiérrez-Millán¹  Jesús Martínez-Barnetche¹  Vianey Saldaña-Navor¹  Federico A. Zumaya-Estrada¹  Mario H. Rodríguez¹  Alejandro Alvarado-Delgado¹  Ángel Tello-López¹  Humberto Lanz-Mendoza² 

[1] Centro de Investigación Sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, Avenida Universidad 655, Santa María Ahuacatitlán, C.P. 62100 Cuernavaca, Morelos, Mexico;Corresponding author.;

关键词: Nyssorhynchus albimanus;    Neuropeptide precursors;    Adipokinetic hormone/corazonin-related peptide;    Hugin;    Myosuppressin;   

DOI  :  

来源: DOAJ

【 摘 要 】

Insect neuropeptides, play a central role in the control of many physiological processes. Based on an analysis of Nyssorhynchus albimanus brain transcriptome a neuropeptide precursor database of the mosquito was described. Also, we observed that adipokinetic hormone/corazonin-related peptide (ACP), hugin and corazonin encoding genes were differentially expressed during Plasmodium infection. Transcriptomic data from Ny. albimanus brain identified 29 pre-propeptides deduced from the sequences that allowed the prediction of at least 60 neuropeptides. The predicted peptides include isoforms of allatostatin C, orcokinin, corazonin, adipokinetic hormone (AKH), SIFamide, capa, hugin, pigment-dispersing factor, adipokinetic hormone/corazonin-related peptide (ACP), tachykinin-related peptide, trissin, neuropeptide F, diuretic hormone 31, bursicon, crustacean cardioactive peptide (CCAP), allatotropin, allatostatin A, ecdysis triggering hormone (ETH), diuretic hormone 44 (Dh44), insulin-like peptides (ILPs) and eclosion hormone (EH). The analysis of the genome of An. albimanus and the generated transcriptome, provided evidence for the identification of myosuppressin neuropeptide precursor. A quantitative analysis documented increased expression of precursors encoding ACP peptide, hugin and corazonin in the mosquito brain after Plasmodium berghei infection. This work represents an initial effort to characterize the neuropeptide precursors repertoire of Ny. albimanus and provides information for understanding neuroregulation of the mosquito response during Plasmodium infection.

【 授权许可】

Unknown