Self-Assembly of Functional Nucleic Acid-Based Colorimetric Competition Assay for the Detection of Immunoglobulin E

Sensors

Shu-Feng Zhou¹  Caiyun Yu¹  Cui Wang¹  Jie Cheng²  Honggui Lin³  Xuexia Lin³  RanjithKumar Kankala³  Jianlong Su⁴ 

[1] Pharmaceutical Engineering, College of Chemical Engineering, Huaqiao University, Xiamen 361021, China;Applied and Environment Microbiology, Department of Biology, Georgie State University, Atlanta, GA 30303, USA;;Department of Chemical Engineering &School of Marine Engineering, Jimei University, Xiamen 361021 China;

关键词: functional nucleic acids;    DNAzyme;    immunoglobulin E;    colorimetric competition detection;   

DOI  :  10.3390/s19102224

来源: DOAJ

【 摘 要 】

In this work, we have developed a simple and rapid colorimetric assay for the detection of immunoglobulin E (IgE) using functional nucleic acids (FNAs) and a solid-phase competition enzyme-linked immunosorbent assay (ELISA). The FNAs including aptamer of recombinant IgE, G-quadruplex and its complementary fragments were immobilized on 96-well microplates to achieve recognition and detection of IgE in biological samples. The G-quadruplex DNAzyme catalyzed 2,2′-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS)-hemin-H₂O₂ system was used to improve the sensitivity of colorimetric assay. In the presence of IgE, the hairpin structure and G-quadruplex would be destroyed, resulting in the inactivation of DNAzyme and subsequent reduction of its absorbance. This cost-effective approach detected IgE in the linear range from 5.0 pg/mL to 500 ng/mL, with the limit of detection (LOD) of 2.0 pg/mL, under optimal conditions. Moreover, the developed method was successfully applied to the rapid detection of IgE in human urine, indicating a great potentiality of this approach in clinical diagnosis and other biomedical applications.

【 授权许可】

Unknown