A Dual Approach of an Oil–Membrane Composite and Boron-Doped Diamond Electrode to Mitigate Biofluid Interferences

Sensors

Michael Brothers¹  Saber Hussain¹  Steve Kim¹  Jeroen van Duren²  Madeleine DeBrosse³  Yuchan Yuan³  Aleksandar Karajic³  Jason Heikenfeld³ 

[1] 711th Human Performance Wing, Air Force Research Laboratory, Wright-Patterson AFB, Dayton, OH 45433, USA;Diamond Foundry Inc., South San Francisco, CA 94080, USA;Novel Device Lab., University of Cincinnati, Cincinnati, OH 45221, USA;

关键词: biosensors;    membranes;    diamond;    redox;    interferents;    foulants;   

DOI  :  10.3390/s21238063

来源: DOAJ

【 摘 要 】

Electrochemical biosensors promise a simple method to measure analytes for both point-of-care diagnostics and continuous, wearable biomarker monitors. In a liquid environment, detecting the analyte of interest must compete with other solutes that impact the background current, such as redox-active molecules, conductivity changes in the biofluid, water electrolysis, and electrode fouling. Multiple methods exist to overcome a few of these challenges, but not a comprehensive solution. Presented here is a combined boron-doped diamond electrode and oil–membrane protection approach that broadly mitigates the impact of biofluid interferents without a biorecognition element. The oil–membrane blocks the majority of interferents in biofluids that are hydrophilic while permitting passage of important hydrophobic analytes such as hormones and drugs. The boron-doped diamond then suppresses water electrolysis current and maintains peak electrochemical performance due to the foulant-mitigation benefits of the oil–membrane protection. Results show up to a 365-fold reduction in detection limits using the boron-doped diamond electrode material alone compared with traditional gold in the buffer. Combining the boron-doped diamond material with the oil–membrane protection scheme maintained these detection limits while exposed to human serum for 18 h.

【 授权许可】

Unknown