Chemical profiling of root bark extract from Oplopanax elatus and its in vitro biotransformation by human intestinal microbiota

PeerJ

Haiqiang Yao¹  Yuqi Wu¹  Wenqian Guo¹  Jing-Xuan Wan¹  Han Ma¹  Xiaolu Wang¹  Jin-Yi Wan¹  Ping Li²  Lian-Wen Qi²  Shilei Wang²  Chong-Zhi Wang³  Chun-Su Yuan³ 

[1] School of Traditional Chinese Medicine & National Institute of TCM Constitution and Preventive Medicine, Beijing University of Chinese Medicine, Beijing, China;State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, China;Tang Center for Herbal Medicine Research & Department of Anesthesia and Critical Care, University of Chicago, Chicago, IL, USA;

关键词: Oplopanax elatus;    Intestinal microbiota;    UPLC-Q-TOF/MS;    Metabolic profiles;    Biotransformation;   

DOI  :  10.7717/peerj.12513

来源: DOAJ

【 摘 要 】

Oplopanax elatus (Nakai) Nakai, in the Araliaceae family, has been used in traditional Chinese medicine (TCM) to treat diseases as an adaptogen for thousands of years. This study established an ultra-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (UPLC-Q-TOF/MS) method to identify chemical components and biotransformation metabolites of root bark extract from O. elatus. A total of 18 compounds were characterized in O. elatus extract, and 62 metabolites by human intestinal microbiota were detected. Two polyynes, falcarindiol and oplopandiol were recognized as the main components of O. elatus, whose metabolites are further illustrated. Several metabolic pathways were proposed to generate the detected metabolites, including methylation, hydrogenation, demethylation, dehydroxylation, and hydroxylation. These findings indicated that intestinal microbiota might play an essential role in mediating the bioactivity of O. elatus.

【 授权许可】

Unknown