Cardioprotection via Metabolism for Rat Heart Preservation Using the High-Pressure Gaseous Mixture of Carbon Monoxide and Oxygen

International Journal of Molecular Sciences

Munekazu Naito¹  Eri Nanizawa¹  Chiharu Suzuki¹  Shun Otsuka¹  Shuichi Hirai¹  Naoyuki Hatayama¹  Hiroshi Seno²  Tadashi Ogawa²  Koichiro Hata³ 

[1] Department of Anatomy, Aichi Medical University, Aichi 480-1195, Japan;Department of Legal Medicine, Aichi Medical University, Aichi 480-1195, Japan;Department of Surgery, Division of Hepato-Biliary-Pancreatic and Transplant Surgery, Graduate School of Medicine, Kyoto University, Kyoto 606-8507, Japan;

关键词: cardioprotection;    ischemic injury;    metabolomics;    carbon monoxide;    oxygen;    high-pressure gas;   

DOI  :  10.3390/ijms21228858

来源: DOAJ

【 摘 要 】

The high-pressure gas (HPG) method with carbon monoxide (CO) and oxygen (O₂) mixture maintains the preserved rat heart function. The metabolites of rat hearts preserved using the HPG method (HPG group) and cold storage (CS) method (CS group) by immersion in a stock solution for 24 h were assessed to confirm CO and O₂ effects. Lactic acid was significantly lower and citric acid was significantly higher in the HPG group than in the CS group. Moreover, adenosine triphosphate (ATP) levels as well as some pentose phosphate pathway (PPP) metabolites and reduced nicotinamide adenine dinucleotide phosphate (NADPH) were significantly higher in the HPG group than in the CS group. Additionally, reduced glutathione (GSH), which protects cells from oxidative stress, was also significantly higher in the HPG group than in the CS group. These results indicated that each gas, CO and O₂, induced the shift from anaerobic to aerobic metabolism, maintaining the energy of ischemic preserved organs, shifting the glucose utilization from glycolysis toward PPP, and reducing oxidative stress. Both CO and O₂ in the HPG method have important effects on the ATP supply and decrease oxidative stress for preventing ischemic injury. The HPG method may be useful for clinical application.

【 授权许可】

Unknown