期刊论文详细信息
Stem Cells Translational Medicine
Human bone marrow stem/stromal cell osteogenesis is regulated via mechanically activated osteocyte‐derived extracellular vesicles
Michele Corrigan1  David A. Hoey1  Kian F. Eichholz1  Marie‐Noelle Labour1  Gillian P. Johnson1  Michelle C. Lowry2  Lorraine O'Driscoll2  Nian Shen3  Ian Woods3  Mathieu Riffault3  Kieran Wynne4 
[1] Department of Mechanical, Aeronautical and Biomedical Engineering Materials and Surface Science Institute, University of Limerick Limerick Ireland;School of Pharmacy and Pharmaceutical Sciences and Trinity Biomedical Sciences Institute Trinity College Dublin Dublin Ireland;Trinity Centre for Biomedical Engineering Trinity Biomedical Sciences Institute, Trinity College Dublin Dublin 2 Ireland;UCD Conway Institute of Biomolecular and Biomedical Research University College Dublin Dublin 4 Ireland;
关键词: bone;    extracellular vesicle;    marrow stem cell;    mechanobiology;    proteomics;   
DOI  :  10.1002/sctm.19-0405
来源: DOAJ
【 摘 要 】

Abstract Bone formation or regeneration requires the recruitment, proliferation, and osteogenic differentiation of stem/stromal progenitor cells. A potent stimulus driving this process is mechanical loading. Osteocytes are mechanosensitive cells that play fundamental roles in coordinating loading‐induced bone formation via the secretion of paracrine factors. However, the exact mechanisms by which osteocytes relay mechanical signals to these progenitor cells are poorly understood. Therefore, this study aimed to demonstrate the potency of the mechanically stimulated osteocyte secretome in driving human bone marrow stem/stromal cell (hMSC) recruitment and differentiation, and characterize the secretome to identify potential factors regulating stem cell behavior and bone mechanobiology. We demonstrate that osteocytes subjected to fluid shear secrete a distinct collection of factors that significantly enhance hMSC recruitment and osteogenesis and demonstrate the key role of extracellular vesicles (EVs) in driving these effects. This demonstrates the pro‐osteogenic potential of osteocyte‐derived mechanically activated extracellular vesicles, which have great potential as a cell‐free therapy to enhance bone regeneration and repair in diseases such as osteoporosis.

【 授权许可】

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