Targeted Methylation of the LncRNA NEAT1 Suppresses Malignancy of Renal Cell Carcinoma

Frontiers in Cell and Developmental Biology

Wanlong Tan¹  Xinhui Liao⁴  Fen Yuan⁴  Jieqing Chen⁴  Jianting Wu⁴  Ganglin Su⁴  Zhongfu Zhang⁴  Hongbing Mei⁴  Jianli Cheng⁴ 

[1] Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou, China;Department of Urology, Shenzhen Second People’s Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, China;Guangdong Key Laboratory of Systems Biology and Synthetic Biology for Urogenital Tumors, Shenzhen Second People’s Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, China;Shenzhen Key Laboratory of Genitourinary Tumor, Shenzhen Second People’s Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, China;

关键词: lncRNA;    NEAT1;    m6A (N6-methyladenosine);    RCC = renal cell cancer;    dCas13b;   

DOI  :  10.3389/fcell.2021.777349

来源: DOAJ

【 摘 要 】

Long-chain non-coding RNA (LncRNA) has been found to play an important role in the regulation of the occurrence and progression of renal cell carcinoma (RCC). In this study, we demonstrated that LncRNA NEAT1 expression and m6A methylation level was decreased in RCC tissues. Further, the downregulated expression level of LncRNA NEAT1 was associated with poor prognosis for RCC patients. Then we used CRIPSR/dCas13b-METTL3 to methylate LncRNA NEAT1 in RCC cells. The results showed that the expression level of LncRNA NEAT1 was upregulated after methylated by dCas13b-METTL3 in RCC cells. And the proliferation and migration ability of RCC cells was decreased after methylated LncRNA NEAT1. Finally, we examined the effect of LncRNA NEAT1 hypermethylation on the transcriptome. We found differentially expressed genes in RCC cells were associated with “cGMP-PKG signaling pathway”, “Cell adhesion molecules” and “Pathways in cancer”. In conclusion, CRISPR/Cas13b-METTL3 targeting LncRNA NEAT1 m6A methylation activates LncRNA NEAT1 expression and provides a new target for treatment of RCC.

【 授权许可】

Unknown