LncRNA NEAT1 facilitates glioma progression via stabilizing PGK1

Journal of Translational Medicine

Dezhi Xu¹  Kang Xie¹  Aimin Li¹  Changtao Liu¹  Jingshan Liang¹ 

[1] Lianyungang Clinical College of Nanjing Medical University/The First People’s Hospital of Lianyungang, No. 182, Tongguan Road, 222000, Lianyungang, China;

关键词: Glioma;    NEAT1;    PGK1;    lncRNA;    Glycolysis;   

DOI  :  10.1186/s12967-022-03273-2

来源: Springer

【 摘 要 】

BackgroundLong noncoding RNA NEAT1 has been implicated in glioma progression. However, the effect of NEAT1 on glycolysis of glioma cell and the potential mechanism remain unclear.MethodsIn vitro experiments, including CCK-8, colony formation, ECAR, and lactate detection assays were performed to evaluate the effect of NEAT1 on proliferation and glycolysis of glioma cell. RNA pulldown and RIP assays were performed to identify the interaction between NEAT1 and PGK1. Truncated mutation of NEAT1 and PGK1 was used to confirm the specific interactive domains between NEAT1 and PGK1. Animal studies were performed to analyze the effect of NEAT1/PGK1 on glioma progression.ResultsNEAT1 knockdown significantly suppressed the proliferation and glycolysis of glioma cells. NEAT1 could specifically interact with PGK1, which promotes PGK1 stability. Hairpin A of NEAT1 is essential for interaction with M1 domain of PGK1. Depletion of NEAT1 markedly inhibited tumor growth in mice, while PGK1 could reverse this effect. Higher expression of NEAT1 was associated with poor overall survival of GBM patients.ConclusionsNEAT1 over expression promotes glioma progression through stabilizing PGK1. NEAT1/PGK1 axis is a candidate therapeutic target for glioma treatment.

【 授权许可】

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