Efficient biodegradation of nitriles by a novel nitrile hydratase derived from Rhodococcus erythropolis CCM2595

Biotechnology & Biotechnological Equipment

Changhai Liang¹  Wenjing Du¹  Jiao Huang²  Yi Guo²  Li Wang²  Baocheng Cui² 

[1] Lab of Advanced Materials and Catalytic Engineering, School of Chemical Engineering, Dalian University of Technology;Lab of Biocalyalysis and Transformation, School of Life and Pharmaceutical Sciences, Dalian University of Technology;

关键词: nitrile hydratase;    purification;    rhodococcus erythropolis ccm2595;    nitriles;   

DOI  :  10.1080/13102818.2021.1941253

来源: DOAJ

【 摘 要 】

In our previous study, we firstly cloned the gene of a novel ReNHase (NHase from Rhodococcus erythropolis CCM2595), and the strain was shown to degrade only phenol, hydroxybenzoate, p-chlorophenol, aniline and other aromatic compounds. Here in, we further purified the ReNHase from R. erythropolis CCM2595 and detected its properties of biodegradation. We constructed a plasmid with the gene of ReNHase with His-tag. The encoding ReNHase was cloned and overexpressed in recombinant Escherihia coli and confirmed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). The substrate scope of the new heterologous ReNHase was tested and the efficiency of degradation of nitriles by the ReNHase was also investigated by high efficiency liquid chromatography. We also studied the effect of temperature and pH on the catalysis of adiponitrile by the purified ReNHase. The recombinant E.coli showed high catalytic regioselectivity with high substrate affinity towards dinitriles (especially for adiponitrile) whereas lower affinity towards mononitriles. Compared to whole-cell catalysis, the catalytic time was shortened significantly with the purified ReNHase. The enzyme activity of crude recombinant E.coli was 635 U g−1 (DCW), while the specific activity of purified ReNHase was 63.107 U mg−1. The apparent Km value for the purified ReNHase is 6.6252 mmol L−1, which revealed the good affinity between the purified ReNHase and adiponitrile. The reaction Kcat is 82.77 s−1 and Kcat/Km is 1.249 × 104 (mol−1 L s−1), which showed high catalytic activity towards adiponitrile. We propose that this purified ReNHase may be applied for the industry and sewage treatment for environmental protection.

【 授权许可】

Unknown