Optimization of the Rheological Properties of Self-Assembled Tripeptide/Alginate/Cellulose Hydrogels for 3D Printing

Polymers

Fouzia Boulmedais¹  Luis Rojo²  María Rosa Aguilar²  Miryam Criado-Gonzalez²  Rebeca Hernández²  Rosa Ana Ramírez-Jiménez²  Alejandro Hernández-Sosa² 

[1] Institut Charles Sadron (UPR 22), Université de Strasbourg, CNRS, 23 rue du Loess, BP 84047, CEDEX 2, 67034 Strasbourg, France;Instituto de Ciencia y Tecnología de Polímeros (ICTP), CSIC, c/Juan de la Cierva, 3, 28006 Madrid, Spain;

关键词: alginate;    hydrogels;    hybrid polymer inks;    peptides;    3D printing;   

DOI  :  10.3390/polym14112229

来源: DOAJ

【 摘 要 】

3D printing is an emerging and powerful technique to create shape-defined three-dimensional structures for tissue engineering applications. Herein, different alginate–cellulose formulations were optimized to be used as printable inks. Alginate (Alg) was chosen as the main component of the scaffold due to its tunable mechanical properties, rapid gelation, and non-toxicity, whereas microcrystalline cellulose (MCC) was added to the hydrogel to modulate its mechanical properties for printing. Additionally, Fmoc-FFY (Fmoc: 9-fluorenylmethoxycarbonyl; F: phenylalanine; Y: tyrosine), a self-assembled peptide that promotes cell adhesion was incorporated into the ink without modifying its rheological properties and shear-thinning behavior. Then, 3D-printed scaffolds made of Alg, 40% of MCC inks and Fmoc-FFY peptide were characterized by scanning electron microscopy and infrared spectroscopy, confirming the morphological microstructure of the hydrogel scaffolds with edged particles of MCC homogeneously distributed within the alginate matrix and the self-assembly of the peptide in a β-sheet conformation. Finally, the cytocompatibility of the scaffolds was tested in contact with the MG63 osteosarcoma cells, confirming the absence of cytotoxic components that may compromise their viability. Interestingly, MG63 cell growth was retarded in the scaffolds containing the peptide, but cells were more likely to promote adhesive interactions with the material rather than with the other cells, indicating the benefits of the peptide in promoting biological functionality to alginate-based biomaterials.

【 授权许可】

Unknown