Alginate Hydrogels Coated with Chitosan for Wound Dressing

Marine Drugs

Maria Cristina Straccia³  Giovanna Gomez d𠆚yala³  Ida Romano¹  Adriana Oliva²  Paola Laurienzo³ 

[1] Institute of Biomolecular Chemistry, CNR, via Campi Flegrei 34, Pozzuoli 80078, Italy; E-Mail:;Department of Biochemistry, Biophysics and General Pathology, Second University of Naples, via L. De Crecchio 7, Naples 80138, Italy; E-Mail:;Institute for Polymers, Composites and Biomaterials (IPCB), CNR, via Campi Flegrei 34, Pozzuoli 80078, Italy; E-Mails:

关键词: chitosan hydrochloride;    alginate;    hydrogels;    antibacterial activity;    sustained release;    wound dressing;   

DOI  :  10.3390/md13052890

来源: mdpi

【 摘 要 】

In this work, a coating of chitosan onto alginate hydrogels was realized using the water-soluble hydrochloride form of chitosan (CH-Cl), with the dual purpose of imparting antibacterial activity and delaying the release of hydrophilic molecules from the alginate matrix. Alginate hydrogels with different calcium contents were prepared by the internal setting method and coated by immersion in a CH-Cl solution. Structural analysis by cryo-scanning electron microscopy was carried out to highlight morphological alterations due to the coating layer. Tests in vitro with human mesenchymal stromal cells (MSC) were assessed to check the absence of toxicity of CH-Cl. Swelling, stability in physiological solution and release characteristics using rhodamine B as the hydrophilic model drug were compared to those of relative uncoated hydrogels. Finally, antibacterial activity against Escherichia coli was tested. Results show that alginate hydrogels coated with chitosan hydrochloride described here can be proposed as a novel medicated dressing by associating intrinsic antimicrobial activity with improved sustained release characteristics.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.