Immunization With Mycobacterium tuberculosis Antigens Encapsulated in Phosphatidylserine Liposomes Improves Protection Afforded by BCG

Frontiers in Immunology

Noemi Poerio¹  Maurizio Fraziano¹  Peter Hart²  Rajko Reljic²  Gil R. Diogo²  Andy C. Tran²  Matthew J. Paul²  Alastair Copland²  Mi-Young Kim²  Mahavir Singh³ 

[1] Dipartimento di Biologia, University of Rome Tor Vergata, Rome, Italy;Institute for Infection and Immunity, St. George's University of London, London, United Kingdom;LIONEX GmbH, Brunswick, Germany;

关键词: liposomes;    tuberculosis;    vaccine;    BCG;    immunity;   

DOI  :  10.3389/fimmu.2019.01349

来源: DOAJ

【 摘 要 】

Liposomes have been long considered as a vaccine delivery system but this technology remains to be fully utilized. Here, we describe a novel liposome-based subunit vaccine formulation for tuberculosis (TB) based on phosphatidylserine encapsulating two prominent TB antigens, Ag85B, and ESAT-6. We show that the resulting liposomes (Lipo-AE) are stable upon storage and can be readily taken up by antigen presenting cells and that their antigenic cargo is delivered and processed within endosomal cell compartments. The Lipo-AE vaccine formulation combined with the PolyIC adjuvant induced a mixed Th1/Th17-Th2 immune response to Ag85B but only a weak response to ESAT-6. An immunization regimen based on systemic delivery followed by mucosal boost with Lipo-AE resulted in the accumulation of resident memory T cells in the lungs. Most importantly though, when Lipo-AE vaccine candidate was administered to BCG-immunized mice subsequently challenged with low dose aerosol Mycobacterium tuberculosis, we observed a significant reduction of the bacterial load in the lungs and spleen compared to BCG alone. We therefore conclude that the immunization with mycobacterial antigens delivered by phosphatidylserine based liposomes in combination with Poly:IC adjuvant may represent a novel BCG boosting vaccination strategy.

【 授权许可】

Unknown