Aptamer-modified Magnetic Nanosensitizer for in vivo MR imaging of HER2-expressing Cancer

Nanoscale Research Letters

Minhee Ku¹  Jung-Hoon Kim¹  Jin-Suck Suh¹  Jaemoon Yang¹  Dan Heo¹ 

[1] Department of Radiology, Yonsei University College of Medicine;

关键词: Magnetic resonance imaging;    Breast cancer;    HER2;    Aptamer;    Contrast agent;    Molecular imaging;   

DOI  :  10.1186/s11671-018-2682-3

来源: DOAJ

【 摘 要 】

Abstract The aim of this study was the development of a human epidermal growth factor receptor 2 (HER2)-targetable contrast agent for magnetic resonance imaging (MRI) with a high magnetic sensitivity. An anti-HER2 aptamer-modified magnetic nanosensitizer (AptHER2-MNS) was prepared by conjugation with 5′-thiol-modified aptamers and maleimidylated magnetic nanocrystals (MNCs). The physicochemical characteristics and targeting ability of AptHER2-MNS were confirmed, and the binding affinity (K d ) onto HER2 protein of AptHER2-MNS was 0.57 ± 0.26 nM. In vivo MRI contrast enhancement ability was also verified at HER2+ cancer cell (NIH3T6.7)-xenograft mouse models (n = 3) at 3T clinical MRI instrument. The control experiment was carried out using non-labeled MNCs. The results indicated that up to 150% contrast enhancement was achieved at the tumor region in the T2-weighted MR images after the injection of the AptHER2-MNS agent in mice that received the NIH3T6.7 cells.

【 授权许可】

Unknown