Development of 18 microsatellite markers for Atractylodes japonica

Applications in Plant Sciences

Mi Ran Kim¹  MyeongWon Oh²  Chun Geon Park²  Jeong‐Hoon Lee²  Jae Ki Chang²  Yun‐Ji Lee²  Jin‐Tae Jeong²  Yi Lee³  Jinsu Gil³  Hee Chung³  Bo‐Keun Ha⁴  Sin‐Gi Park⁵  Chang Pyo Hong⁵ 

[1] Department of Bioresources Foundation of Agri, Tech, Commercialization and Transfer (FACT) Iksan 54672 Republic of Korea;Department of Herbal Crop Research National Institute of Horticultural and Herbal Science (NIHHS) Rural Development Administration Eumseong 27709 Republic of Korea;Department of Industrial Plant Science and Technology Chungbuk National University Cheongju 28644 Republic of Korea;Division of Plant Biotechnology Chonnam National University Gwangju 61186 Republic of Korea;Theragen Etex Bio Institute Suwon 16229 Republic of Korea;

关键词: Asteraceae;    Atractylodes japonica;    genetic diversity;    microsatellite markers;    population genetics;    simple sequence repeat (SSR);   

DOI  :  10.1002/aps3.11350

来源: DOAJ

【 摘 要 】

Premise Atractylodes japonica (Asteraceae) is endemic to East Asia, where its rhizomes are used in traditional medicine. To investigate the genetic diversity of this species, we developed polymorphic microsatellite markers. Methods and Results We obtained a total of 175,825 simple sequence repeat (SSR) loci using the Illumina HiSeq 2500 system. Eighteen polymorphic SSR primer pairs were selected to determine heterozygosity levels and allele numbers in 80 individuals from four A. japonica populations. The levels of observed and expected heterozygosity ranged from 0.000 to 1.000 and from 0.133 to 0.892, respectively. Cross‐amplification in the related species A. macrocephala and A. lancea was successful in 15 and 14 of the 18 markers, respectively. Conclusions These microsatellite markers will be useful for future studies involving A. japonica population genetics and breeding.

【 授权许可】

Unknown