Genetic Variability of Macedonian Tobacco Varieties Determined by Microsatellite Marker Analysis

Diversity

Katarina Davalieva²  Ivana Maleva²  Kiril Filiposki¹  Ognen Spiroski² 

[1] University “St Kliment Ohridski”, Bitola, Tobacco Institute, Kicevski pat bb, 7500 Prilep, Republic of Macedonia; E-Mail:;Research Centre for Genetic Engineering and Biotechnology, Macedonian Academy of Sciences and Arts, Krste Misirkov 2, POB 428, 1000 Skopje, Republic of Macedonia; E-Mails:

关键词: Nicotiana tabacum L;    PCR;    microsatellite markers;    tobacco varieties;    genetic diversity;   

DOI  :  10.3390/d2040439

来源: mdpi

【 摘 要 】

Tobacco (Nicotiana tabacum L.) is an important agricultural crop plant for the economy of many countries. Assessment of the genetic diversity of cultivated tobacco varieties is of importance for long-term tobacco improvement. Microsatellite markers are currently the marker system of choice for genetic analysis of allopolyploid plants. In this study, we evaluated the use of 30 microsatellite markers for identification of 10 varieties of cultivated tobacco in the Republic of Macedonia. We found 24 of the microsatellite markers to be polymorphic and sufficient for identification of these varieties. Cluster analysis showed that Macedonian tobacco varieties are classifiable into three distinct groups.

【 授权许可】

CC BY   
© 2010 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.