Complete Mitochondrial Genome Sequence and Identification of a Candidate Gene Responsible for Cytoplasmic Male Sterility in Celery (Apium graveolens L.)

International Journal of Molecular Sciences

Jinkui Liu¹  Liang Sun¹  Qing Cheng¹  Yingxue Zhang¹  Huolin Shen¹  Tiantian Li¹  Yihao Wang¹  Peng Wang² 

[1] Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, Department of Vegetable Science, College of Horticulture, China Agricultural University, Beijing 100193, China;Institute of Vegetable Research, Guangxi Academy of Agricultural Sciences, Nanning 530007, China;

关键词: celery;    cytoplasmic male sterility (CMS);    mitochondrial genome;    CMS-associated gene;   

DOI  :  10.3390/ijms22168584

来源: DOAJ

【 摘 要 】

Celery (Apium graveolens L.) is an important leafy vegetable worldwide. The development of F₁ hybrids in celery is highly dependent on cytoplasmic male sterility (CMS) because emasculation is difficult. In this study, we first report a celery CMS, which was found in a high-generation inbred line population of the Chinese celery “tanzhixiangqin”. Comparative analysis, following sequencing and assembly of the complete mitochondrial genome sequences for this celery CMS line and its maintainer line, revealed that there are 21 unique regions in the celery CMS line and these unique regions contain 15 ORFs. Among these ORFs, only orf768a is a chimeric gene, consisting of 1497 bp sequences of the cox1 gene and 810 bp unidentified sequences located in the unique region, and the predicted protein product of orf768a possesses 11 transmembrane domains. In summary, the results of this study indicate that orf768a is likely to be a strong candidate gene for CMS induction in celery. In addition, orf768a can be a co-segregate marker, which can be used to screen CMS in celery.

【 授权许可】

Unknown