Transcriptome and Hormone Comparison of Three Cytoplasmic Male Sterile Systems in Brassica napus

International Journal of Molecular Sciences

Hui Wang¹  Shifei Sang¹  Mengyu Hao¹  Wenxiang Wang¹  Bingli Ding¹  Qiong Hu¹  QamarU Zaman¹  Desheng Mei¹  Li Fu¹  Hongtao Cheng¹ 

[1] Key Laboratory for Biological Sciences and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China;

关键词: cytoplasmic male sterility (CMS);    phytohormones;    differentially expressed genes;    pollen development;    Brassica napus;   

DOI  :  10.3390/ijms19124022

来源: DOAJ

【 摘 要 】

The interaction between plant mitochondria and the nucleus markedly influences stress responses and morphological features, including growth and development. An important example of this interaction is cytoplasmic male sterility (CMS), which results in plants producing non-functional pollen. In current research work, we compared the phenotypic differences in floral buds of different Brassica napus CMS (Polima, Ogura, Nsa) lines with their corresponding maintainer lines. By comparing anther developmental stages between CMS and maintainer lines, we identified that in the Nsa CMS line abnormality occurred at the tetrad stage of pollen development. Phytohormone assays demonstrated that IAA content decreased in sterile lines as compared to maintainer lines, while the total hormone content was increased two-fold in the S₂ stage compared with the S₁ stage. ABA content was higher in the S₁ stage and exhibited a two-fold decreasing trend in S₂ stage. Sterile lines however, had increased ABA content at both stages compared with the corresponding maintainer lines. Through transcriptome sequencing, we compared differentially expressed unigenes in sterile and maintainer lines at both (S₁ and S₂) developmental stages. We also explored the co-expressed genes of the three sterile lines in the two stages and classified these genes by gene function. By analyzing transcriptome data and validating by RT-PCR, it was shown that some transcription factors (TFs) and hormone-related genes were weakly or not expressed in the sterile lines. This research work provides preliminary identification of the pollen abortion stage in Nsa CMS line. Our focus on genes specifically expressed in sterile lines may be useful to understand the regulation of CMS.

【 授权许可】

Unknown