Methicillin-resistant Staphylococcus aureus in cows with mastitis, the presence of the mecA gene and the gene for virulence

Mljekarstvo

Željko Cvetnić¹  Boris Habrun¹  Manuela Zadravec²  Darko Majnarić³  Vesna Jaki Tkalec³  Jadranka Jurmanović³  Branka Šeol Martinec⁴ 

[1] Hrvatski veterinarski institut, Odjel za bakteriologiju i parazitologiju, Savska cesta 143, 10000 Zagreb, Hrvatska;Hrvatski veterinarski institut, Odjel za veterinarsko javno zdravstvo, Savska cesta 143, 10000 Zagreb, Hrvatska;Hrvatski veterinarski institut, Veterinarski zavod Križevci, Zakmardijeva 10, 48000 Križevci, Hrvatska;Veterinarski fakultet, Zavod za mikrobiologiju i zarazne bolesti s klinikom, Heinzelova 55, 10000 Zagreb, Hrvatska;

关键词: mastitic cows;    Staphylococcus aureus;    virulence genes;    mecA gene;   

DOI  :  10.15567/mljekarstvo.2015.0406

来源: DOAJ

【 摘 要 】

The physiological properties of 47 Staphylococcus aureus strains were investigated. The test strains were grown on bacteriological media and identified by the ID32 STAF system for biochemical identification of bacteria. Sensitivity to antimicrobial agents was performed by the disc diffusion method. The nuc gene and the virulence factors coa, hla, hlb, hld, hlg, hlg-2, tst, eta, etb, lukF-PV and lukS-PV and mecA gene were detected by the polymerase chain reaction. Furthermore, the spa type of the studied isolates was also set. According to the obtained results, all strains had the nuc, coa, hla and hld gene. Ten strains (21.3 %) had also the tst gene, while 37 strains (78.7 %) had the hlg gene and 35 strains (74.5 %) had the hlb and hlg-2 genes. All of the investigated S. aureus isolates were penicillin resistant (100 %), with 29 strains which were also resistant to oxacillin (61.7 %). Methicillin (oxacillin) resistance was detected by the mecA gene detection, which is also the first MRSA result from the secretion samples of cows’ mammary glands in Croatia. The researched MRSA strains proved to belong to different spa types, and the most common were spa types t005, t011 and t521, and a new spa type t9498 was detected.

【 授权许可】

Unknown