Quality evaluation of the extract of aerial parts from Atractylodes lancea based on fingerprint and chemometrics

International Journal of Food Properties

Xiaofeng Dai¹  Yuefei Liu¹  Fangfang Pan²  Juan Yang³  Ziying Wang³  Zhongyuan Li³  Xiumei Li⁴  Wei Tian⁵  Peilong Yang⁵ 

[1] Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, HE, China;D Department, Inner Mongolia Jiuhe Agricultural Science and Technology Development Co. Ltd Hulunbeier, NM, China;Key Laboratory of Feed Biotechnology, Ministry of Agriculture and Rural Affairs, Institute of Feed Research of Caas, BJ, China;;R&;State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Industrial Fermentation Microbiology of Ministry of Education &

关键词: Atractylodes lancea;    Antibacterial activity;    Chemometrics;    Similarity analysis;    High-performance liquid chromatography;   

DOI  :  10.1080/10942912.2022.2046052

来源: DOAJ

【 摘 要 】

The high-performance liquid chromatography (HPLC) fingerprint technique is an effective method to evaluate the quality, identify species, ensure consistency and stability of traditional Chinese medicine (TCM). In this study, determination was performed on an Agilent ZORBAX SB-C18 (4.6 × 250 mm, 5 µm) column with methanol-water as mobile phase in gradient elution at the flow rate of 0.8 mL/min, the column temperature was 30°C, the detection wavelength was set to 260 nm. The HPLC fingerprints of 10 batches of extract of the aerial parts from Atractylodes lancea were evaluated by similarity analysis and chemometrics including hierarchical cluster analysis, principal component analysis. Meanwhile, the antibacterial activities of 10 batches of extract of the aerial parts from Atractylodes lancea were estimated on Escherichia coli (E. coli). The correlation coefficient of similarity between 10 batches of extract was ≥ 0.998, and the extract of the aerial parts from Atractylodes lancea have stable effects on inhibiting the proliferation of E. coli. Besides, bivariate correlation analysis (BCA) and partial least squares regression (PLSR) were applied to construct a spectrum-effect relationship, which proved the 5 main marker compounds were more relevant to anti-bacteria. Therefore, in this work, the results were successfully built an agile and efficient method that can ensure the intra-assay stability of biological activity of the extract of the aerial parts from Atractylodes lancea.

【 授权许可】

Unknown