Conjugates of Chitosan and Calcium Alginate with Oligoproline and Oligohydroxyproline Derivatives for Potential Use in Regenerative Medicine

Materials

Sandra Flinčec Grgac¹  Anita Tarbuk¹  Eulalia Gliscinska²  Zbigniew Draczynski²  Marta Kaminska³  Mariusz Dudek³  Zbigniew J. Kaminski⁴  Beata Kolesinska⁴  Justyna Fraczyk⁴  Angelika Becht⁴  Joanna Wasko⁴ 

[1] Department of Textile Chemistry and Ecology, University of Zagreb Faculty of Textile Technology, Prilaz baruna Filipovića 28a, HR-10000 Zagreb, Croatia;Institute of Material Sciences of Textiles and Polymer Composites, Faculty of Material Technologies and Textile Design, Lodz University of Technology, Zeromskiego 116, 90-924 Lodz, Poland;Institute of Materials Science and Engineering, Faculty of Mechanical Engineering, Lodz University of Technology, Stefanowskiego 1/15, 90-924 Lodz, Poland;Institute of Organic Chemistry, Faculty of Chemistry, Lodz University of Technology, Zeromskiego 116, 90-924 Lodz, Poland;

关键词: conjugate of polysaccharides with peptides;    alginate;    chitosan;    proline;    hydroxyproline derivatives;    mimetic of functional collagen fragments;   

DOI  :  10.3390/ma13143079

来源: DOAJ

【 摘 要 】

New materials that are as similar as possible in terms of structure and biology to the extracellular matrix (external environment) of cells are of great interest for regenerative medicine. Oligoproline and oligohydroxyproline derivatives (peptides 2–5) are potential mimetics of collagen fragments. Peptides 2–5 have been shown to be similar to the model collagen fragment (H-Gly-Hyp-Pro-Ala-Hyp-Pro-OH, 1) in terms of both their spatial structure and biological activity. In this study, peptides 2–5 were covalently bound to nonwovens based on chitosan and calcium alginate. Incorporation of the peptides was confirmed by Fourier transform -infrared (FT-IR) and zeta potential measurements. Biological studies (cell metabolic activity by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test and Live/Dead assay) proved that the obtained peptide-polysaccharide conjugates were not toxic to the endothelial cell line EA.hy 926. In many cases, the conjugates had a highly affirmative influence on cell proliferation. The results of this study show that conjugates of chitosan and calcium alginate with oligoproline and oligohydroxyproline derivatives have potential for use in regenerative medicine.

【 授权许可】

Unknown