Chitosan-Alginate Biocomposite Containing Fucoidan for Bone Tissue Engineering

Marine Drugs

Jayachandran Venkatesan¹  Ira Bhatnagar² 

[1] Marine Bioprocess Research Center, Department of Chemistry, Pukyong National University, Busan 608-737, Korea; E-Mail:;Nanotheranostics Laboratory, Centre for Cellular and Molecular Biology, Hyderabad 500-007, India; E-Mail:

关键词: chitosan;    alginate;    fucoidan;    bone tissue engineering;    biomaterials;   

DOI  :  10.3390/md12010300

来源: mdpi

【 摘 要 】

Over the last few years, significant research has been conducted in the construction of artificial bone scaffolds. In the present study, different types of polymer scaffolds, such as chitosan-alginate (Chi-Alg) and chitosan-alginate with fucoidan (Chi-Alg-fucoidan), were developed by a freeze-drying method, and each was characterized as a bone graft substitute. The porosity, water uptake and retention ability of the prepared scaffolds showed similar efficacy. The pore size of the Chi-Alg and Chi-Alg-fucoidan scaffolds were measured from scanning electron microscopy and found to be 62–490 and 56–437 µm, respectively. In vitro studies using the MG-63 cell line revealed profound cytocompatibility, increased cell proliferation and enhanced alkaline phosphatase secretion in the Chi-Alg-fucoidan scaffold compared to the Chi-Alg scaffold. Further, protein adsorption and mineralization were about two times greater in the Chi-Alg-fucoidan scaffold than the Chi-Alg scaffold. Hence, we suggest that Chi-Alg-fucoidan will be a promising biomaterial for bone tissue regeneration.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.