Fe2+ Ions Alleviate the Symptom of Citrus Greening Disease

International Journal of Molecular Sciences

Motofumi Suzuki¹  Tomoya Matsuyama¹  Sakiko Yamashita-Muraki²  Hiroki Ono²  Yoshikuni Masaoka²  Kayoko Honda²  Kanako Fujiwara²  Shoji Sugano³  Haruhiko Inoue³  Takamasa Nonaka⁴  Yuichi Kato⁴ 

[1] Environment and Energy Innovation department, Frontier Research and Development Division, Aichi steel Corporation, Wanowari, Arao-machi, Tokai, Aichi 476-8666, Japan;Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima 739-8528, Japan;Plant Function Research Unit, Division of Plant and Microbial Sciences, National Agriculture and Food Research Organization (NARO), Institute of Agrobiological Sciences,Tsukuba, Ibaraki 305-8605, Japan;Toyota Central R&D Labs., Inc., Yokomichi, Nagakute, Aichi 480-1192, Japan;

关键词: citrus greening;    huanglongbing;    iron nutrient;    PCR;    Ferroptosis;   

DOI  :  10.3390/ijms21114033

来源: DOAJ

【 摘 要 】

Citrus greening (CG) is among the most devastating citrus diseases worldwide. CG-infected trees exhibit interveinal chlorotic leaves due to iron (Fe) deficiency derived from CG; thus, Fe content is lower in infected leaves than in healthy leaves. In this study, we demonstrated that the foliar application of Fe²⁺ relieves the symptom of CG infection in citrus trees. We applied Fe²⁺ and citrate to the leaves of infected rough lemon plants. Following this treatment, a reduction in the number of yellow symptomatic leaves was observed, and their growth was restored. Using chlorophyll content as an index, we screened for effective Fe complexes and found that a high ratio of citrate to Fe²⁺ in the applied solution led to effects against CG in Shikuwasa trees. A high proportion of Fe²⁺ to total Fe was another key factor explaining the effectiveness of the solution in CG infection, indicating the importance of Fe²⁺ absorption into plant cells. We confirmed the proportion of Fe²⁺ to total Fe through the high correlation of reflectometry data via a triazine reaction and X-ray absorption fine structure analysis. These results demonstrate that the foliar application of a high-Fe²⁺ citrate solution can restore the growth of CG diseased trees.

【 授权许可】

Unknown