Effect of Fluvastatin on Oxidation-reduction Function and ICAM-1 Expression in Pocine Carotid Artery Endothelial Cells Dealt with Hypoxia/Reoxygenation

Journal of International Translational Medicine

Kayoko Gomita¹  Toshiyuki Hagiwara¹  Fukuda Daiju²  Sata Masataka² 

[1] Department of Cardiology, Tokyo Women’s Medical University, Tokyo, Japan;Department of Cardiology, University of Tokyo Graduate School of Medicine, Tokyo, Japan;

关键词: Fluvastatin;    Endothelial cells;    Carotid artery;    Hypoxia/reoxygenation;    Pocine;    Intercellular adhesion molecule-1;   

DOI  :  10.11910/2227-6394.2014.02.04.01

来源: DOAJ

【 摘 要 】

Objective: To explore the protective function of fluvastatin on endothelial cells in an ischemiareperfusion process. Methods: Pocine carotid artery endothelial cells (PCAEC) were cultured, grown together with different concentrations of fluvastatin (0.1 μmol/L, 0.2 μmol/L, 0.5 μmol/L, 1.0 μmol/L) for 44 h, and then divided into normal control group, different concentrations of fluvastatin groups and H/R group. Serum immunology and cell immunochemistry were used to detect the levels of methyl thiazolyl tetrazolium (MTT), glutathione peroxidase (GSH-PX), malondialdehyde (MDA), superoxide dismutase (SOD) and intercellular adhesion molecule-1 (ICAM-1) after 1-h hypoxia and 3-h reoxygenation. The effect of fluvastatin on oxidation-reduction function and ICAM-1 expression in PCAEC dealt with hypoxia/reoxygenation (H/R) was observed. Results: There was significant difference regarding the cell viability between H/R group intervened by 0.5 μmol/L of fluvastatin and simple H/R group (P＝0.01). H/R could obviously decrease SOD activity in culture cells, and the generated MDA was conspicuously higher by comparison to fluvastatin group and normal control group (P＝0.001). Significant differences were presented regarding GSH-PX level between normal control group, fluvastatin group and H/R group (P＝0.002). Additionally, ICAM-1 cell immunochemical staining showed marked differences among each group (P＝0.018). Conclusion: Proper concentration of fluvastatin can protect H/R endothelial cells.

【 授权许可】

Unknown