期刊论文详细信息
Nanomaterials
Preparation of Dual-Layered Core–Shell Fe3O4@SiO2 Nanoparticles and Their Properties of Plasmid DNA Purification
Gye Seok An1  Jin Soon Han2 
[1] Department of Advanced Material Engineering, Kyonggi University, Suwon 16227, Korea;Division of Materials Science and Engineering, Hanyang University, Seoul 04763, Korea;
关键词: Fe3O4;    nanocomposites;    magnetic properties;    SiO2;    plasmid DNA purification;   
DOI  :  10.3390/nano11123422
来源: DOAJ
【 摘 要 】

The rapid purification of biomaterials such as DNA, RNA, and antibodies has attracted extensive attention, and research interest has increased further with the COVID-19 pandemic. In particular, core–shell-structured superparamagnetic nanoparticles have been continuously studied for their application as biopurification materials. It has been reported that Fe3O4@SiO2 nanoparticles are one of the most promising candidates for separating nucleic acids via a simple and rapid process. This study proposed a fabrication method for dual-layered Fe3O4@SiO2 nanoparticles, in which the density of the SiO2 shell was controlled using an intermediate surfactant during the SiO2 coating. After the fabrication of dual-layered Fe3O4@SiO2 nanoparticles, structural, morphological, and magnetic analyses were conducted. The results showed that the Fe3O4 nanoparticles were surrounded by a dense layer 15.6~27.9 nm thick and a porous layer 24.2~44.4 nm thick, and had superparamagnetic properties with high saturated magnetization at room temperature (86.9 emu/g). Then, the optimal conditions for the biopurification material were suggested based on analysis of the selective separation of plasmid DNA.

【 授权许可】

Unknown   

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