Detection of Chlamydia trachomatis in Pap Smear Samples from South Khorasan Province of Iran

International Journal of Fertility and Sterility

Malaknaz Ghannadkafi¹  Masood Ziaee²  Mohammad Hasan Namaei²  Davod Javanmard²  Mahmoodreza Behravan²  Alireza Salehabadi² 

[1] Faulty of Nursing and Midwifery, Birjand University of Medical Sciences, Birjand, Iran;Infectious Diseases Research Center, Birjand University of Medical Sciences, Birjand, Iran;

关键词: cervical cancer;    iran;    pap smear;    sexually transmitted infection;   

DOI  :  10.22074/ijfs.2018.5064

来源: DOAJ

【 摘 要 】

BackgroundChlamydia trachomatis (CT), the most common bacterial sexually transmitted infection (STI), leadsto pelvic inflammatory disease, infertility and chronic pelvic pain in women as well as an increased risk of verticaltransmission, conjunctivitis and pneumonitis in infants. It may also be a co-factor along with human papillomavirus(HPV) in cervical cancer progression. We aimed to determine the prevalence of CT genotypes in genital specimens ofwomen from South Khorasan, Iran and to test the association between CT and cytology statistics.Materials and MethodsThis was a cross-sectional study on 248 Pap smear samples from women who visited agynecologist for routine Pap smear testing in South Khorasan province. Nested polymerase chain reaction (PCR) wasused to test the residual fluids of Pap smears for CT-DNA after cytological examination. Direct sequencing, alignmentand phylogenic analyses were performed on eight samples to identify their genotypes.ResultsThe mean age of patients was 37.54 ± 5.21 years. Most samples had a normal cytology (214 cases, 86.29%).Overall, 31 samples were positive for CT infection (12.5%) of which 20 (9.34%) were normal and 11 (32.35%) wereabnormal, with the frequency difference being significant (P=0.022). The co-infection of CT/HPV in total was identified in 14 cases (5.6%). The results of sequencing eight samples out of the 31 CT positive samples revealed the detection of genotypes D and E, each with four cases.ConclusionWe show that a high prevalence of genital CT infection is present in women with both normal and abnormal cytology; however, the higher prevalence among women in the abnormal group may indicate its involvement incervical neoplasia.

【 授权许可】

Unknown