Differential cytotoxicity of long-chain bases for human oral gingival epithelial keratinocytes, oral fibroblasts, and dendritic cells

Data in Brief

Katherine S. Walters¹  Janet M. Guthmiller²  Joseph E. Cavanaugh³  Emily A. Lanzel⁴  Christopher Poulsen⁵  Georgia K. Johnson⁵  Leslie A. Mehalick⁵  Kim A. Brogden⁵  Amber M. Bates⁶  Carol L. Fischer⁶  Philip W. Wertz⁶ 

[1]Central Microscopy Research Facility, The University of Iowa, Iowa City, IA 52242, USA

[2]College of Dentistry, University of Nebraska Medical Center, Lincoln, NE 68583, USA

[3]Department of Biostatistics, College of Public Health, The University of Iowa, Iowa City, IA 52242, USA

[4]Department of Oral Pathology, Radiology and Medicine, College of Dentistry, The University of Iowa, Iowa City, IA 52242, USA

[5]Department of Periodontics, College of Dentistry, The University of Iowa, Iowa City, IA 52242, USA

[6]Dows Institute for Dental Research, College of Dentistry, The University of Iowa, Iowa City, IA 52242, USA

关键词: Cytotoxicity;    Human oral gingival epithelial keratinocytes;    Oral gingival fibroblasts;    Dendritic cells;    Oral squamous cell carcinoma cells;    Sphingosine;    Dihydrosphingosine;    Phytosphingosine;    Glycerol monolaurate;   

DOI  :  10.1016/j.dib.2015.08.025

来源: DOAJ

【 摘 要 】

Long-chain bases, found in the oral cavity, have potent antimicrobial activity against oral pathogens. In an article associated with this dataset, Poulson and colleagues determined the cytotoxicities of long-chain bases (sphingosine, dihydrosphingosine, and phytosphingosine) for human oral gingival epithelial (GE) keratinocytes, oral gingival fibroblasts (GF), dendritic cells (DC), and squamous cell carcinoma (SCC) cell lines [1]. Poulson and colleagues found that GE keratinocytes were more resistant to long-chain bases as compared to GF, DC, and SCC cell lines [1]. In this study, we assess the susceptibility of DC to lower concentrations of long chain bases. 0.2–10.0 µM long-chain bases and GML were not cytotoxic to DC; 40.0–80.0 µM long-chain bases, but not GML, were cytotoxic for DC; and 80.0 µM long-chain bases were cytotoxic to DC and induced cellular damage and death in less than 20 mins. Overall, the LD50 of long-chain bases for GE keratinocytes, GF, and DC were considerably higher than their minimal inhibitory concentrations for oral pathogens, a finding important to pursuing their future potential in treating periodontal and oral infections.

【 授权许可】

Unknown