Effects of the Ayurved Siriraj Wattana recipe on functional and phenotypic characterization of cytokine-induced killer cells and dendritic cells in vitro

BMC Complementary and Alternative Medicine

Research Article

Tawee Laohapan¹  Khanit Sa-ngiamsuntorn²  Kittipong Maneechotesuwan³  Kanda Kasetsinsombat⁴  Sunisa Duangsa-ard⁴  Adisak Wongkajornsilp⁴  Nuntarak Numchaisermsuk⁴  Valla Wamanuttajinda⁴  Pravit Akarasereenont⁵ 

[1] Center of Applied Thai Traditional Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, 10700, Bangkok, Thailand;Department of Biochemistry, Faculty of Pharmacy, Mahidol University, 10400, Bangkok, Thailand;Department of Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, 2 Wanglang Road, 10700, Bangkoknoi, Bangkok, Thailand;Department of Pharmacology, Faculty of Medicine Siriraj Hospital, Mahidol University, 10700, Bangkok, Thailand;Department of Pharmacology, Faculty of Medicine Siriraj Hospital, Mahidol University, 10700, Bangkok, Thailand;Center of Applied Thai Traditional Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, 10700, Bangkok, Thailand;

关键词: CIK cells;    Dendritic cells;    Lymphocytes;    Traditional medicine Asia;    Immunomodulation;    Cytotoxicity;   

DOI  :  10.1186/s12906-016-1480-7

 received in 2016-07-14, accepted in 2016-11-10,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundAyurved Siriraj Wattana recipe (AVS073), has been prescribed as tonic, to increase appetite, and for pain relief. It also exhibits antioxidant, anti-inflammatory, immunomodulating and anti-cancer activities. However, the immunomodulatory effects on antigen-presenting cells and effector T cells remained elusive. We thus aimed to study the effects of AVS073 on differentiation, maturation, functions and proportions of CIK cells and monocyte-derived DCs.MethodsCIK cells and monocyte-derived DCs were treated with AVS073, followed by the assessment of T-helper (Th) phenotypes using real-time RT-PCR and flow cytometry.ResultsAVS073 promoted Th1 phenotype in CD3+CD56+ subset of CIK cells through increasing STAT4, T-bet, and interferon-γ. AVS073 inhibited Th2 phenotype through decreasing STAT6. AVS073 inhibited Treg phenotype through decreasing STAT5A, STAT5B and IDO. AVS073 promoted Th17 phenotype through increasing STAT3, RORC and IL-17. AVS073 treatment of mDCs resulted in increasing Th1-prone cytokine (IL-12) and Th17-prone cytokines (IL-6 and IL-23).ConclusionsAVS073 upregulated Th1 and Th17, but downregulated Th2 and Treg phenotypes within CD3+CD56+ cells. The treatment of mDCs drove Th1 and Th17-polarizations.

【 授权许可】

CC BY   
© The Author(s). 2016