Efficacy of Bacterial Cellulose as a Carrier of BMP-2 for Bone Regeneration in a Rabbit Frontal Sinus Model

Materials

Takaya Yamada¹  Takashi Koike²  Katsumi Hideshima²  Jingjing Sha²  Takahiro Kanno²  Yunpeng Bai²  Yuhei Matsuda² 

[1]Depart of Experimental Animals, Interdisciplinary Center for Science Research, Organization for Research, Shimane University, Izumo, Shimane 693-8501, Japan

[2]Department of Oral and Maxillofacial Surgery, Shimane University Faculty of Medicine, 89-1 Enya-Cho, Izumo, Shimane 693-8501, Japan

关键词: bacterial cellulose;    bone morphogenetic protein-2;    osteocalcin;    proliferating cell nuclear antigen;    sinus lift;    bone regeneration;   

DOI  :  10.3390/ma12152489

来源: DOAJ

【 摘 要 】

If the alveolar bone height of patients requiring dental implants in the maxillary molar region is inadequate, it is difficult to achieve satisfactory outcomes using existing bone graft materials. We previously reported the possible utility of bacterial cellulose (BC) as a new dental treatment material. BC has a high absorptive capacity, good mechanical strength, and good volume retention. BC loaded with bone morphogenetic protein-2 (BMP-2) might allow effective alveolar bone augmentation. We created critical frontal bone defect models in 12 male Japanese white rabbits and divided them into four groups: sham; BC (BC grafting only); BMP-2 (treated with BMP-2 solution only); and BC+BMP-2 (grafted with BC loaded with BMP-2). Newly formed bone volume was calculated via hematoxylin-eosin staining evaluation. The proliferating cell nuclear antigen and osteocalcin levels were determined by the immunohistochemical staining analysis. All measured indices of the BC+BMP-2 group were significantly superior to those of the other groups (all p < 0.05). BC maintained the graft space and released BMP-2 in a sustained manner, promoting optimal bone formation. The BC+BMP-2 combination enhanced bone regeneration and shows promise as a useful means of clinical pre-dental implant bone augmentation in the maxillary sinus.

【 授权许可】

Unknown