Regulation of biomaterial implantation-induced fibrin deposition to immunological functions of dendritic cells

Materials Today Bio

Lina Liu¹  Yi Jia¹  Yun Wang¹  Fuzhou Tang¹  Zhu Zeng¹  Jin Chen²  Zuquan Hu²  Wei Qiu²  Shi Zhou²  Peng Yu³  Wenhui Hu³  Jinhua Long³  Jing Zhou³  Yuannong Ye³ 

[1] Engineering, Guizhou Medical University, Guiyang, 550025, PR China;Key Laboratory of Infectious Immunity and Antibody Engineering in Guizhou Province, Guiyang, 550025, PR China;;School of Basic Medical Sciences / School of Biology &

关键词: Biomaterial implantation;    Fibrin;    Dendritic cells;    Immunological functions;    Biomechanics;    Mechanobiology;   

DOI  :  

来源: DOAJ

【 摘 要 】

The performance of implanted biomaterials is largely determined by their interaction with the host immune system. As a fibrous-like 3D network, fibrin matrix formed at the interfaces of tissue and material, whose effects on dendritic cells (DCs) remain unknown. Here, a bone plates implantation model was developed to evaluate the fibrin matrix deposition and DCs recruitment in vivo. The DCs responses to fibrin matrix were further analyzed by a 2D and 3D fibrin matrix model in vitro. In vivo results indicated that large amount of fibrin matrix deposited on the interface between the tissue and bone plates, where DCs were recruited. Subsequent in vitro testing denoted that DCs underwent significant shape deformation and cytoskeleton reorganization, as well as mechanical property alteration. Furthermore, the immune function of imDCs and mDCs were negatively and positively regulated, respectively. The underlying mechano-immunology coupling mechanisms involved RhoA and CDC42 signaling pathways. These results suggested that fibrin plays a key role in regulating DCs immunological behaviors, providing a valuable immunomodulatory strategy for tissue healing, regeneration and implantation.

【 授权许可】

Unknown