Updating MISEV: Evolving the minimal requirements for studies of extracellular vesicles

Journal of Extracellular Vesicles

Sophie Rome¹  Susmita Sahoo²  Andrew F Hill³  Edit I Buzás⁴  Lei Zheng⁵  Uta Erdbrügger⁶  Cherie Blenkiron⁷  Takahiro Ochiya⁸  Deborah CI Goberdhan⁹  Dolores Di Vizio^1,10  Kenneth W Witwer^1,11  Qing‐Ling Fu^1,12  Juan M Falcón‐Pérez^1,13  Clotilde Théry^1,14  Metka Lenassi^1,15  Jan Lötvall^1,16  Rienk Nieuwland^1,17  Lorraine O'Driscoll^1,18  Joshua A Welsh^1,19 

[1] CarMeN Laboratory (INSERM 1060, INRAE 1397) University of Lyon & Faculty of Medicine Lyon‐Sud Pierre‐Bénite France;Cardiovascular Research Institute Icahn School of Medicine at Mount Sinai New York New York USA;Department of Biochemistry and Genetics La Trobe Institute for Molecular Science La Trobe University Bundoora VIC Australia;Department of Genetics Cell‐ and Immunobiology HCEMM‐SU Extracellular Vesicles Research Group, and ELKH‐SE Immune‐Proteogenomics Extracellular Vesicles Research Group Semmelweis University Budapest Hungary;Department of Laboratory Medicine Nanfang Hospital Southern Medical University Guangzhou China;Department of Medicine Division of Nephrology University of Virginia Charlottesville Virginia USA;Department of Molecular Medicine and Pathology The University of Auckland Auckland New Zealand;Department of Molecular and Cellular Medicine Tokyo Medical University Tokyo Japan;Department of Physiology Anatomy and Genetics University of Oxford Oxford UK;Department of Surgery Department of Pathology & Laboratory Medicine Samuel Oschin Comprehensive Cancer Institute Cedars‐Sinai Medical Center Division of Cancer Biology and Therapeutics Los Angeles California USA;Departments of Molecular and Comparative Pathobiology and Neurology and The Richman Family Precision Medicine Center of Excellence in Alzheimer's Disease Johns Hopkins University School of Medicine Baltimore Maryland USA;Exosome Research and Translational Center The First Affiliated Hospital Otorhinolaryngology Hospital Sun Yat‐sen University Guangzhou China;Exosomes Laboratory & Metabolomics Platf CIC bioGUNE‐BRTA IKERBASQUE, CIBERehd Bilbao Spain;INSERM U932, Institut Curie PSL Research University Paris France;Institute of Biochemistry and Molecular Genetics Faculty of Medicine University of Ljubljana Ljubljana Slovenia;Krefting Research Centre University of Gothenburg Göteborg Sweden;Laboratory of Experimental Clinical Chemistry and Vesicle Observation Center Amsterdam University Medical Center University of Amsterdam Amsterdam The Netherlands;School of Pharmacy and Pharmaceutical Sciences Panoz Institute and Trinity Biomedical Sciences Institute (TBSI) & Trinity St. James's Cancer Institute (TSJCI) Trinity College Dublin Dublin Ireland;Translational Nanobiology Section Laboratory of Pathology Center for Cancer Research National Cancer Institute National Institutes of Health Bethesda Maryland USA;

关键词: ectosomes;    exosomes;    extracellular vesicles;    microvesicles;    MISEV;    reproducibility;   

DOI  :  10.1002/jev2.12182

来源: DOAJ

【 摘 要 】

Abstract The minimal information for studies of extracellular vesicles (EVs, MISEV) is a field‐consensus rigour initiative of the International Society for Extracellular Vesicles (ISEV). The last update to MISEV, MISEV2018, was informed by input from more than 400 scientists and made recommendations in the six broad topics of EV nomenclature, sample collection and pre‐processing, EV separation and concentration, characterization, functional studies, and reporting requirements/exceptions. To gather opinions on MISEV and ideas for new updates, the ISEV Board of Directors canvassed previous MISEV authors and society members. Here, we share conclusions that are relevant to the ongoing evolution of the MISEV initiative and other ISEV rigour and standardization efforts.

【 授权许可】

Unknown