Functional classification and biochemical characterization of a novel rho class glutathioneS‐transferase inSynechocystis PCC 6803

FEBS Open Bio

Arvind Kumar Singh¹  Gaurav Chhetri¹  Timir Tripathi¹  Ramesh Chinta¹  Tripti Pandey¹  Bijay Kumar¹  Dev Bukhsh Singh² 

[1] Department of Biochemistry, North-Eastern Hill University, Shillong 793022, India;Department of Biotechnology, Institute of Biosciences and Biotechnology, Chhatrapati Shahu Ji Maharaj University, Kanpur 208024, India;

关键词: Cyanobacteria;    Detoxification;    Glutathione;    Dichloroacetate;    Water pollutant;    Bioremediation;   

DOI  :  10.1016/j.fob.2014.11.006

来源: DOAJ

【 摘 要 】

We report a novel class of glutathioneS‐transferase (GST) from the model cyanobacteriumSynechocystis PCC 6803 (sll1545) which catalyzes the detoxification of the water pollutant dichloroacetate and also shows strong glutathione‐dependent peroxidase activity representing the classical activities of zeta and theta/alpha class respectively. Interestingly, sll1545 has very low sequence and structural similarity with these classes. This is the first report of dichloroacetate degradation activity by any bacterial GST. Based on these results we classify sll1545 to a novel GST class, rho. The present data also indicate potential biotechnological and industrial applications of cyanobacterial GST in dichloroacetate‐polluted areas.

【 授权许可】

Unknown