Российский паразитологический журнал | |
GENOTOXIC AND CYTOTOXIC EFFECTS IN HOST CELLS AT TAENIIDOSIS | |
V. V. Zorina1  V. Ya. Bekish1  | |
[1] Vitebsk State Medical University, Belarus210023, Belarus, Vitebsk, 27 Frunze prsp.; | |
关键词: taenia solium; t. saginatus; gel electrophoresis; genome damage; genotoxic and cytotoxic effects; golden hamster; | |
DOI : | |
来源: DOAJ |
【 摘 要 】
Objective of research: The pork and beef tapeworm infestation is characterized by the increase of single-strand breaks, alkali-labile sites in nuclear DNA of bone marrow cells of golden hamsters. A triple subdermal sensibilisation with protein somatic products from the tissue of Taenia solium or Taeniarinchus saginatus is accompanied by a genotoxic effect in somatic bone marrow cells and generative cells in mouse seminal vesicles. It is manifested by increased single-strand breaks and alkali-labile sites in a nuclear DNA. The increase of damage of nuclear DNA depends on the dose of protein somatic products from the tissue of T. saginatus, it authentically grows up when a higher dose is used. Protein somatic products from the tissue of T. solium or T. saginatus at subdermal sensibilisation have a cytotoxic effect manifested in growth of apoptotic cells in bone marrow and seminal vesicles. Materials and methods. To study the genotoxic and cytotoxic effects in genome of mammals at taenia infection and at triple subdermal sensibilisation with protein somatic products from the tissue of T. solium or T. saginatus the alkaline single cell gel electrophoresis by Singh et al., modified by Hellman et al. (comet) assay is used for investigation of bone marrow and seminal vesicles. Results and discussion. The values of the alkaline single cell gel electrophoresis (comet) assay of bone marrow and seminal vesicles at T. solium or T. saginatus infection in golden hamsters were determined on 45th day of infestation.
【 授权许可】
Unknown