The Investigation of Alpha-Tubulin Differential Expression in Oligodendroglioma Brain Tumor Aiming MALDI-TOF-TOF

International Clinical Neuroscience Journal

Saeed Hesami Tackallou¹  Mehdi Pooladi²  Soheila Karani²  Mehrdad Hashemi³ 

[1] Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran;Department of Biology, School of Basic Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran;Department of Molecular Genetics, Tehran Medical Branch, Islamic Azad University, Tehran, Iran;

关键词: Alpha-Tubulin;    Oligodendroglioma;    MALDI-TOF-TOF;    Proteomics;   

DOI  :  10.15171/icnj.2019.11

来源: DOAJ

【 摘 要 】

Background: Tubulin is known as a heterodimer protein, which includes alpha and beta tubulin subunits. This structural protein plays important roles in pathogenesis and healing different diseases. Biomarkers help in fast and accurate detection of cancer. Proteomic studies can be useful both in biological and clinical research, also help obtain protein expression profiles by using twodimensional electrophoresis, mass spectrometry, and bioinformatics tools. Finding candidate proteins as cancer biomarkers is an interesting area in proteomic investigations.Methods: In the present study, the total protein content of healthy cells of the brain and brain tumor cells were extracted, purified and quantified by Bradford assay. Two-dimensional electrophoresis used for protein separation followed by statistical analysis. Primary protein detection was performed based on the differences in isoelectric pH, the molecular weight of proteins and protein data banks, which was further confirmed by Matrix Assisted Laser Desorption Ionisation-Time-of-Flight (MALDITOF-TOF).Results: In this study, an alpha-tubulin expression found changed (overexpression) in Oligodendroglioma tumors comparing control identified by proteomics analysis. Also, alphatubulin position showed in the oligodendroglioma tumors cluster diagram. Conclusion: Proteome analysis approach has allowed biology and medical studies. Alpha-tubulin introduced as a candidate biomarker for the diagnosis and prediction of oligodendroglioma tumors.

【 授权许可】

Unknown