Polydopamine-Assisted Silver Nanoparticle Self-Assembly on Sericin/Agar Film for Potential Wound Dressing Application

International Journal of Molecular Sciences

Peng Wang¹  Rui Cai¹  Hua Zuo²  Huawei He³  Lisha Ai³  Gang Tao³  Ping Zhao³  Liying Liu³  Yejing Wang³  Meirong Yang³ 

[1] College of Biotechnology, Southwest University, Chongqing 400715, China;College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China;State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China;

关键词: polydopamine;    silver nanoparticle;    sericin;    antimicrobial activity;    cytocompatibility;   

DOI  :  10.3390/ijms19102875

来源: DOAJ

【 摘 要 】

Silver nanoparticles (AgNPs) are extensively applied for their broad-spectrum and excellent antibacterial ability in recent years. Polydopamine (PDA) has great advantages for synthesizing large amounts of AgNPs, as it has multiple sites for silver ion binding and phenolic hydroxyl structure to reduce silver ions to AgNPs. Here, we mixed sericin and agar solution and dried at 65 °C to prepare a sericin (SS)/Agar composite film, and then coated polydopamine (PDA) on the surface of SS/Agar film by soaking SS/Agar film into polydopamine solution, subsequently synthesizing high-density AgNPs with the assistance of PDA to yield antibacterial AgNPs-PDA- SS/Agar film. Scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FT-IR) and X-ray diffraction (XRD) spectra indicated the successful synthesis of high-density AgNPs on the surface of PDA-SS/Agar film. PDA coating and AgNPs modification did not affect the structure of sericin and agar. Furthermore, water contact angle, water absorption and mechanical property analysis showed that AgNPs-PDA-SS/Agar film had excellent hydrophilicity and proper mechanical properties. Inhibition zone and growth curve assays suggested the prepared film had excellent and long-lasting antibacterial ability. In addition, it had excellent cytocompatibility on the fibroblast NIH/3T3 cells. The film shows great potential as a novel kind of wound dressing.

【 授权许可】

Unknown