Journal of Applied Animal Research | |
Comparison of single radial immunodiffusion and ELISA for the quantification of immunoglobulin G in bovine colostrum, milk and calf sera | |
Steven Morrison1  Amanda Dunn1  Alan Gordon1  Bernadette Earley2  Anastasio Argűello2  Michael Welsh3  Catherine Duffy4  | |
[1] Agri-food and Bio-sciences Institute;Animal and Grassland Research and Innovation Centre (AGRIC), Teagasc, Grange;Sisaf;Veterinary Science Division; | |
关键词: ELISA; single radial immunodiffusion; zinc sulphate turbidity test; Brix refractometer; IgG; calf; colostrum; | |
DOI : 10.1080/09712119.2017.1394860 | |
来源: DOAJ |
【 摘 要 】
The overall objective was to compare immunoglobulin G (IgG) concentrations measured by single radial immunodiffusion (sRID) and ELISA-based methods in samples of bovine colostrum and transition milk from contrasting breed types (Limousin × Friesian (n = 10) and Holstein (n = 10)). Jugular blood samples were collected at 48 h post-birth from beef (n = 10) and dairy (n = 10) calves and sera harvested subsequent to colostrum consumption. Absolute colostrum IgG values determined by ELISA showed poor agreement with mean (SD) IgG values measured using sRID, fixed bias (sRID – ELISA) was 31.89 (±9.84) mg/mL; having wide limits of agreement (12.61–51.17) and a low concordance coefficient (0.26). The agreement between ELISA and sRID when measuring serum IgG was greater than that of colostrum, fixed bias (sRID – ELISA) was 12.36 (±6.60) mg/mL; having narrower limits of agreement (−0.58 to 25.30) and serum IgG concentrations had a greater concordance coefficient (0.44) between samples. Calf sera IgG measured using the indirect zinc sulphate turbidity test showed a strong correlation with the sRID and ELISA methods (P < .001), R2 = 0.78 and R2 = 0.77 respectively. Overall, the ELISA and sRID methodologies had a strong positive association with almost a twofold (1.8) difference between values; therefore, they provide diverse absolute values of IgG concentration.
【 授权许可】
Unknown