Occurrence of potentially toxigenic mould species in fresh salads of different kinds of ready-for-use vegetables

Acta Periodica Technologica

关键词: toxigenic moulds;    mycotoxins;    fresh salads;    vegetables;   

DOI  :  10.2298/APT1041033K

来源: DOAJ

【 摘 要 】

In the mycological survey of fresh salads of different vegetables, thecollected samples were tested for total counts of moulds with specialattention paid to the presence of potentially toxigenic species. The surveyalso included the isolation and the identification of species, as well as theevaluation of mycotoxin biosynthesis ability of potential producers ofochratoxin A (OA) and sterigmatocystin (STC). Mould counts ranged from 10.0to 4.7x102 cfu g-1. The most common moulds found in fresh salads wereCladosporium (42.89%), Penicillium (25.78%), Aspergillus (14.67%) andAlternaria (6.89%). C. cladosporioides (40.44%), followed by A. niger(10.22%), P. aurantiogriseum (7.55%), A. alternata (6.89%) and Fusarium spp.(3.11%) were the most dominating species. Other species were represented with2.22% (Eurotium spp.), 1.56% (Botrytis spp.), 0.67% (Phoma spp.), 0.44%(Geotrichum spp., Mucor spp., Phialophora spp.) and 0.22% (Emericella spp.,Paecilomyces spp., Trichoderma spp., Xeromyces spp.). Twenty-two of 41identified mould species were potentially toxigenic, which accounted for46.18% of the total isolated population. The most frequent were the potentialproducers of ochratoxin A (17.77%). Potential producers of moniliformin wereisolated in 3.11% of samples, while producers of fumonisin and STC were foundin 2.67% and 2.44% of samples, respectively. The tested isolates of OAproducers did not demonstrate the ability to biosynthetise this mycotoxins,but two out of five isolates of A. versicolor were found to biosynthesise STCin doses of 109.2 ng mL-1 and 56.3 ng mL-1. The obtained results indicatethat such products may threaten human health, considering that isolatedspecies were potentially toxigenic, while isolates of A. versicolor alsobiosynthesised STS.

【 授权许可】

Unknown