Ex Vivo Generation and Characterization of Human Hyaline and Elastic Cartilaginous Microtissues for Tissue Engineering Applications

Biomedicines

Rik Verplancke¹  Jan Vanfleteren¹  Víctor Carriel²  Fernando Campos²  David Sánchez-Porras²  Jesús Chato-Astrain²  Daniel Durand-Herrera²  ÓscarDarío García-García²  JoséDarío Sánchez-López³  AnaB. Paes⁴ 

[1] Centre for Microsystems Technology (CMST), imec and Ghent University, 9052 Ghent, Belgium;Department of Histology, Tissue Engineering Group, Faculty of Medicine, University of Granada, 18016 Granada, Spain;Division of Maxillofacial Surgery, University Hospital Complex of Granada, 18013 Granada, Spain;Master Program in Tissue Engineering and Advanced Therapies, International School for Postgraduate Studies, University of Granada, 18016 Granada, Spain;

关键词: microtissues;    tissue engineering;    human hyaline chondrocytes;    human elastic chondrocytes;    extracellular matrix;    organoids;   

DOI  :  10.3390/biomedicines9030292

来源: DOAJ

【 摘 要 】

Considering the high prevalence of cartilage-associated pathologies, low self-repair capacity and limitations of current repair techniques, tissue engineering (TE) strategies have emerged as a promising alternative in this field. Three-dimensional culture techniques have gained attention in recent years, showing their ability to provide the most biomimetic environment for the cells under culture conditions, enabling the cells to fabricate natural, 3D functional microtissues (MTs). In this sense, the aim of this study was to generate, characterize and compare scaffold-free human hyaline and elastic cartilage-derived MTs (HC-MTs and EC-MTs, respectively) under expansion (EM) and chondrogenic media (CM). MTs were generated by using agarose microchips and evaluated ex vivo for 28 days. The MTs generated were subjected to morphometric assessment and cell viability, metabolic activity and histological analyses. Results suggest that the use of CM improves the biomimicry of the MTs obtained in terms of morphology, viability and extracellular matrix (ECM) synthesis with respect to the use of EM. Moreover, the overall results indicate a faster and more sensitive response of the EC-derived cells to the use of CM as compared to HC chondrocytes. Finally, future preclinical in vivo studies are still needed to determine the potential clinical usefulness of these novel advanced therapy products.

【 授权许可】

Unknown