Physical optimization of cell proliferation and differentiation using spinner flask and microcarriers

AMB Express

Haijuan Hu¹  Shilei Li¹  Yushuang Li²  Feng Yang²  Yingying Li²  Wenting Liu²  Shouwei Wang² 

[1] Beijing Institute of Food Science;China Meat Research Center;

关键词: Cell proliferation;    Cell-based meat;    Myogenesis;    Spontaneous contraction;    Microcarriers;    Spinner flask;   

DOI  :  10.1186/s13568-022-01397-8

来源: DOAJ

【 摘 要 】

Abstract The traditional breeding industry has been increasingly saturated and caused environmental pollution, disease transmission, excessive resource use, and methane emission; however, it still cannot meet the needs of the growing population. To explore other alternatives, researchers focused on cell agriculture and cell-based meat, especially large-scale cell culture. As a prerequisite for production, large-scale culture technology has become an important bottleneck restricting cell-based meat industrialization. In this study, the single-factor variable method was adopted to examine the influence of Cytodex1 microcarrier pretreatment, spinner flask reaction vessel, cell culture medium, serum and cell incubation, and other influencing factors on large-scale cell cultures to identify the optimization parameters suitable for 3D culture environment. Collagen and 3D culture were also prospectively explored to promote myogenesis and cultivate tissue-like muscle fibers that contract spontaneously. This research lays a theoretical foundation and an exploratory practice for large-scale cell cultures and provides a study reference for the microenvironment of myoblast culture in vitro, a feasible direction for the cell therapy of muscular dystrophy, and prerequisites for the industrialized manufacturing of cell-based meat. Graphical Abstract Graphical summary: Research on large-scale myoblast culture using spinner flasks and microcarriers. For cell culture, the microcarriers were pretreated with UV and collagen. Cell seeding condition, spinner flask speed, resting time, and spinner flask culture microenvironment were then optimized. Finally, two culture systems were prepared: a culture system based on large-scale cell expansion and a culture system for myogenesis promotion and differentiation

【 授权许可】

Unknown