Creation of cotton mutant library based on linear electron accelerator radiation mutation

Biochemistry and Biophysics Reports

Xuwu Sun¹  Jiajing Wang²  Zihao Zhao²  Yixin Zhang²  Zhixin Liu²  Yaping Zhou²  Rui Wu²  Chenxi Guo²  Xiaole Yu²  Aizhi Qin²  George Bawa² 

[1] Corresponding author. State Key Laboratory of Cotton Biology, Henan University, 85 Minglun Street, Kaifeng, 475001, China.;State Key Laboratory of Cotton Biology, State Key Laboratory of Crop Stress Adaptation and Improvement, Key Laboratory of Plant Stress Biology, School of Life Sciences, Henan University, 85 Minglun Street, Kaifeng, 475001, China;

关键词: Cotton;    Linear electron accelerator;    Radiation mutagenesis;    Mutant library;    Mutant;   

DOI  :  

来源: DOAJ

【 摘 要 】

Cotton (Gossypium spp.) is one of the most important cash crops worldwide. At present, new cotton varieties are mainly produced through conventional cross breeding, which is limited by available germplasm. Although the genome of cotton has been fully sequenced, research on the function of specific genes lags behind due to the lack of sufficient genetic material. Therefore, it is very important to create a cotton mutant library to create new, higher-quality varieties and identify genes associated with the regulation of key traits. Traditional mutagenic strategies, such as physical, chemical, and site-directed mutagenesis, are relatively costly, inefficient, and difficult to perform. In this study, we used a radiation mutation method based on linear electron acceleration to mutate cotton variety ‘TM-1’, for which a whole-genome sequence has previously been performed, to create a high throughput cotton mutant library. Abundant phenotypic variation was observed in the progeny population for three consecutive generations, including cotton fiber color variation, plant dwarfing, significant improvement of yield traits, and increased sensitivity to Verticillium wilt. These results show that radiation mutagenesis is an effective and feasible method to create plant mutant libraries.

【 授权许可】

Unknown