Resveratrol activates the histone H2B ubiquitin ligase, RNF20, in MDA-MB-231 breast cancer cells

Journal of Functional Foods

Wei-Chun Hsiao¹  Cheng-Fu Kao²  Chia-Yeh Lin²  Duncan Edward Wright³  Chia-Ling Hsu³  Yi-Chen Lo³  Guoo-Shyng Wang Hsu⁴ 

[1] Institute of Cellular and Organismic Biology, Academia Sinica, 128 Sec2, Academia Road, Nankang, Taipei 11529, Taiwan;Graduate Institute of Nutrition and Food Sciences, Fu-Jen Catholic University, No. 510, Zhongzheng Road, Xinzhuang Dist., New Taipei City 24205, Taiwan;Institute of Cellular and Organismic Biology, Academia Sinica, 128 Sec2, Academia Road, Nankang, Taipei 11529, Taiwan;Institute of Food Science and Technology, National Taiwan University, No. 1, Sec 4, Roosevelt Road, Taipei 10617, Taiwan;

关键词: Resveratrol;    RNF20;    H2B ubiquitylation;    Breast cancer;    p21;    Cell growth;   

DOI  :  

来源: DOAJ

【 摘 要 】

Resveratrol, a polyphenolic compound found in grapes, has been shown to partially exert its anti-cancer function through the modulation of epigenetic events. However, the epigenetic targets of resveratrol in breast cancer are not yet fully understood. Here, we identified that the gene encoding the histone H2B ubiquitin ligase RNF20 (ring finger protein 20), a chromatin modifying enzyme and putative tumour suppressor, is up-regulated by resveratrol in MDA-MB-231 breast cancer cells. Up-regulation of RNF20 is achieved through an increase of active histone marks around its promoter. The increase in RNF20 in MDA-MB-231 likely contributes to p21 regulation and the anti-growth effects of resveratrol, as RNF20 knockdown diminished these effects. Our findings suggest that RNF20 is a novel epigenetic target of resveratrol, and increase our understanding of how it contributes to the chemopreventive effect of resveratrol in a more aggressive breast cancer cell line, MDA-MB-231.

【 授权许可】

Unknown