Biosynthesis of organic molecules via artificial cascade reactions based on cytochrome P450 monooxygenases

Green Synthesis and Catalysis

Aitao Li¹  Carlos G. Acevedo-Rocha²  Jing Zhao³  Ren-Jie Li³  Zhongwei Zhang³ 

[1] Corresponding authors.;Biosyntia ApS, Copenhagen 2100, Denmark;State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei Key Laboratory of Industrial Biotechnology, School of Life Sciences, Hubei University, Wuhan 430062, China;

关键词: Cascade reaction;    P450 monooxygenase;    Oxyfunctionalization;    Biocatalysis;    Artificial pathway;   

DOI  :  

来源: DOAJ

【 摘 要 】

Cytochrome P450 monooxygenases (P450s) play crucial roles in the oxyfunctionalization of non-activated hydrocarbons, thus bridging the gap between simple molecules and high value-added fine chemicals. The introduction of P450s into artificially designed cascade reactions provides an exciting opportunity to accomplish challenging reactions and access organic compounds that cannot be achieved by traditional chemical catalysts or by natural metabolic pathways. The main objective of this review is to provide an overview of different types of artificially designed multi-step cascades in which P450s are involved as key catalysts in the biosynthesis of various organic molecules. The different efforts include in vitro multi-enzymatic biocatalytic cascades, in vivo biocatalytic cascades as well as chemo-enzymatic hybrid cascades. Overall, this work provides an overview of cascade reactions involving P450s with various potential applications for the industrial production of food, cosmetics, polymers and pharmaceuticals.

【 授权许可】

Unknown