Basic Apoptotic Mechanisms of Lead Toxicity in Human Leukemia (Hl-60) Cells

International Journal of Environmental Research and Public Health

关键词: lead nitrate;    HL-60 cells;    annexin V;    caspase-3;    flow cytometry;    apoptosis;   

DOI  :  10.3390/ijerph7052008

来源: DOAJ

【 摘 要 】

Lead exposure represents a medical and public health emergency, especially in children consuming high amounts of lead-contaminated flake paints. It may also cause hematological effects to people of all ages. Recent studies in our laboratory have indicated that apoptosis may be associated with the lead-induced oxidative stress and DNA damage. However, the mechanisms underlying its effect on lymphocytes are still largely unknown. Therefore, the aim of the present study was to investigate the apoptotic mechanisms of lead nitrate [Pb(NO3)2] using HL-60 cells as a test model. HL-60 cells were treated with different concentrations of Pb(NO3)2 for 24 h prior to cell viability assay and flow cytometry assessment. The results obtained from the trypan blue exclusion test indicated that at very low concentration, Pb(NO3)2 has no effect on the viability of HL-60 cells. A significant (p

【 授权许可】

Unknown