International Journal of Environmental Research and Public Health | |
Basic Apoptotic Mechanisms of Lead Toxicity in Human Leukemia (Hl-60) Cells | |
Clement G. Yedjou1  Jessica N. Milner2  Carolyn B. Howard2  | |
[1] Cellomics and Toxicogenomics Research Laboratory, NIH-Center for Environmental Health, College of Science, Engineering and Technology, Jackson State University, 1400 Lynch Street, P.O. Box 18540, Jackson, MS 39217, USA; | |
关键词: lead nitrate; HL-60 cells; annexin V; caspase-3; flow cytometry; apoptosis; | |
DOI : 10.3390/ijerph7052008 | |
来源: mdpi | |
【 摘 要 】
Lead exposure represents a medical and public health emergency, especially in children consuming high amounts of lead-contaminated flake paints. It may also cause hematological effects to people of all ages. Recent studies in our laboratory have indicated that apoptosis may be associated with the lead-induced oxidative stress and DNA damage. However, the mechanisms underlying its effect on lymphocytes are still largely unknown. Therefore, the aim of the present study was to investigate the apoptotic mechanisms of lead nitrate [Pb(NO3)2] using HL-60 cells as a test model. HL-60 cells were treated with different concentrations of Pb(NO3)2 for 24 h prior to cell viability assay and flow cytometry assessment. The results obtained from the trypan blue exclusion test indicated that at very low concentration, Pb(NO3)2 has no effect on the viability of HL-60 cells. A significant (
【 授权许可】
CC BY
© 2010 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.
【 预 览 】
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RO202003190053819ZK.pdf | 532KB | download |